| Title | The E3 ubiquitin ligase activity of RING1B is not essential for early mouse development. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | Illingworth R, Moffat M, Mann AR, Read D, Hunter CJ, Pradeepa MM, Adams IR, Bickmore WA |
| Journal | Genes Dev |
| Volume | 29 |
| Issue | 18 |
| Pagination | 1897-902 |
| Date Published | 2015 Sep 15 |
| ISSN | 1549-5477 |
| Keywords | Animals, Embryo, Mammalian, Gene Expression Regulation, Developmental, Histones, Mice, Mice, Inbred C57BL, Mouse Embryonic Stem Cells, Mutation, Polycomb Repressive Complex 1, Polycomb Repressive Complex 2, Protein Binding, Ubiquitin-Protein Ligases, Ubiquitination |
| Abstract | Polycomb-repressive complex 1 (PRC1) and PRC2 maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. However, it is still unclear how they are targeted and how they function. We show that the ability of RING1B, a core component of PRC1, to ubiquitinate histone H2A is dispensable for early mouse embryonic development and much of the gene repression activity of PRC1. Our data support a model in which PRC1 and PRC2 reinforce each other's binding but suggest that the key functions of PRC1 lie beyond the enzymatic capabilities of RING1B. |
| DOI | 10.1101/gad.268151.115 |
| Alternate Journal | Genes Dev. |
| PubMed ID | 26385961 |
| PubMed Central ID | PMC4579347 |
| Grant List | MC_PC_U127527202 / / Medical Research Council / United Kingdom MC_PC_U127580973 / / Medical Research Council / United Kingdom / / Medical Research Council / United Kingdom |
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