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In situ hybridization of corticotropin-releasing factor-encoding messenger RNA in the hypothalamus of the white sucker, Catostomus commersoni.

TitleIn situ hybridization of corticotropin-releasing factor-encoding messenger RNA in the hypothalamus of the white sucker, Catostomus commersoni.
Publication TypeJournal Article
Year of Publication1992
AuthorsOkawara Y, Ko D, Morley SD, Richter D, Lederis KP
JournalCell Tissue Res
Volume267
Issue3
Pagination545-9
Date Published1992 Mar
ISSN0302-766X
KeywordsAnimals, Autoradiography, Base Sequence, Corticotropin-Releasing Hormone, Female, Fishes, Hypothalamus, Immune Sera, Immunohistochemistry, Male, Molecular Sequence Data, Neurons, Nucleic Acid Hybridization, Oligonucleotide Probes, Phosphorus Radioisotopes, Preoptic Area, RNA, Messenger
Abstract

In situ hybridization procedure with a 32P-labelled synthetic oligonucleotide probe was used to detect corticotropin-releasing factor-encoding messenger RNA (CRF mRNA) in the hypothalamus of the white sucker, Catostomus commersoni. Adjacent sections were immunostained by a sucker CRF-specific antiserum. CRF mRNA-containing neurons were identified by autoradiography in the magnocellular and parvocellular subdivisions of the preoptic nucleus (PON). Many of these neurons were also immunostained by sucker antiserum, showing the same distribution patterns. These results confirm the presence of CRF mRNA and CRF peptide in the white sucker hypothalamus and support the view that the magnocellular and parvocellular neurons of the PON may be involved in the control of adrenocorticotropic hormone secretion from the pituitary in the white sucker.

Alternate JournalCell Tissue Res.
PubMed ID1571966