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Prospective identification of myogenic endothelial cells in human skeletal muscle.

TitleProspective identification of myogenic endothelial cells in human skeletal muscle.
Publication TypeJournal Article
Year of Publication2007
AuthorsZheng B, Cao B, Crisan M, Sun B, Li G, Logar A, Yap S, Pollett JB, Drowley L, Cassino T, Gharaibeh B, Deasy BM, Huard J, Péault B
JournalNat Biotechnol
Date Published2007 Sep
KeywordsAdolescent, Adult, Aged, Animals, Antigens, CD56, Biomarkers, Cell Proliferation, Cell Survival, Cells, Cultured, Child, Clone Cells, Endothelial Cells, Flow Cytometry, Humans, Mice, Mice, SCID, Middle Aged, Muscle, Skeletal, Neoplasms, Regeneration, Time Factors

We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease.

Alternate JournalNat. Biotechnol.
PubMed ID17767154
Grant List1U54 AR 050733-01 / AR / NIAMS NIH HHS / United States
R01 AR 049684 / AR / NIAMS NIH HHS / United States
R01 DE 13420-06 / DE / NIDCR NIH HHS / United States
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