|Title||Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015).|
|Publication Type||Journal Article|
|Year of Publication||2015|
|Authors||Villarin BLucendo, Cameron K, Szkolnicka D, Rashidi H., Bates N, Kimber SJ, Flint O, Forbes SJ, Iredale JP, Bradley M, Hay DC|
|Journal||Adv Healthc Mater|
|Date Published||2015 Aug|
One major obstacle to the routine deployment of stem cell-derived cells is their instability in culture. On page 1820 David C. Hay and co-workers describe the use of a synthetic polymer surface. The image shows stem cell-derived hepatocytes replated on this polyurethane surface. Importantly the cells express Zonal Occludin (green stain) at the cell surface, which indicates that the cells display elements of polarization. The blue stain is DAPI, which demarks the nucleus.
|Alternate Journal||Adv Healthc Mater|