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The polycomb-group gene Ezh2 is required for early mouse development.

TitleThe polycomb-group gene Ezh2 is required for early mouse development.
Publication TypeJournal Article
Year of Publication2001
AuthorsO'Carroll D, Erhardt S, Pagani M, Barton SC, Surani MA, Jenuwein T
JournalMol Cell Biol
Date Published2001 Jul
KeywordsAnimals, Blastocyst, Crosses, Genetic, Drosophila Proteins, Embryo Implantation, Embryonic and Fetal Development, Female, Gastrula, Gene Targeting, Humans, In Situ Hybridization, Male, Mice, Mice, Transgenic, Multigene Family, Nuclear Proteins, Polycomb Repressive Complex 2, Pregnancy, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells

Polycomb-group (Pc-G) genes are required for the stable repression of the homeotic selector genes and other developmentally regulated genes, presumably through the modulation of chromatin domains. Among the Drosophila Pc-G genes, Enhancer of zeste [E(z)] merits special consideration since it represents one of the Pc-G genes most conserved through evolution. In addition, the E(Z) protein family contains the SET domain, which has recently been linked with histone methyltransferase (HMTase) activity. Although E(Z)-related proteins have not (yet) been directly associated with HMTase activity, mammalian Ezh2 is a member of a histone deacetylase complex. To investigate its in vivo function, we generated mice deficient for Ezh2. The Ezh2 null mutation results in lethality at early stages of mouse development. Ezh2 mutant mice either cease developing after implantation or initiate but fail to complete gastrulation. Moreover, Ezh2-deficient blastocysts display an impaired potential for outgrowth, preventing the establishment of Ezh2-null embryonic stem cells. Interestingly, Ezh2 is up-regulated upon fertilization and remains highly expressed at the preimplantation stages of mouse development. Together, these data suggest an essential role for Ezh2 during early mouse development and genetically link Ezh2 with eed and YY1, the only other early-acting Pc-G genes.

Alternate JournalMol. Cell. Biol.
PubMed ID11390661
PubMed Central IDPMC87093
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