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A mutant defective in sexual development produces aseptate ascogonia.

TitleA mutant defective in sexual development produces aseptate ascogonia.
Publication TypeJournal Article
Year of Publication2010
AuthorsBloemendal S, Lord KM, Rech C, Hoff B, Engh I, Read ND, Kück U
JournalEukaryot Cell
Volume9
Issue12
Pagination1856-66
Date Published2010 Dec
ISSN1535-9786
KeywordsAmino Acid Sequence, Fruiting Bodies, Fungal, Fungal Proteins, Gene Expression Regulation, Developmental, Gene Expression Regulation, Fungal, Hyphae, Molecular Sequence Data, Mutation, Sequence Alignment, Sordariales
Abstract

The transition from the vegetative to the sexual cycle in filamentous ascomycetes is initiated with the formation of ascogonia. Here, we describe a novel type of sterile mutant from Sordaria macrospora with a defect in ascogonial septum formation. This mutant, named pro22, produces only small, defective protoperithecia and carries a point mutation in a gene encoding a protein that is highly conserved throughout eukaryotes. Sequence analyses revealed three putative transmembrane domains and a C-terminal domain of unknown function. Live-cell imaging showed that PRO22 is predominantly localized in the dynamic tubular and vesicular vacuolar network of the peripheral colony region close to growing hyphal tips and in ascogonia; it is absent from the large spherical vacuoles in the vegetative hyphae of the subperipheral region of the colony. This points to a specific role of PRO22 in the tubular and vesicular vacuolar network, and the loss of intercalary septation in ascogonia suggests that PRO22 functions during the initiation of sexual development.

DOI10.1128/EC.00186-10
Alternate JournalEukaryotic Cell
PubMed ID20952581
PubMed Central IDPMC3008273
Grant ListBB/E010741/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
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