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Metalloprotease-disintegrin ADAM8: expression analysis and targeted deletion in mice.

TitleMetalloprotease-disintegrin ADAM8: expression analysis and targeted deletion in mice.
Publication TypeJournal Article
Year of Publication2005
AuthorsKelly K, Hutchinson G, Nebenuis-Oosthuizen D, Smith AG, Bartsch JW, Horiuchi K, Rittger A, Manova K, Docherty AJP, Blobel CP
JournalDev Dyn
Date Published2005 Jan
KeywordsADAM Proteins, Alleles, Animals, Antigens, CD, beta-Galactosidase, Blotting, Western, Bone and Bones, Bone Development, Female, Gene Deletion, Gene Expression Regulation, Developmental, Genetic Vectors, Image Processing, Computer-Assisted, Immunohistochemistry, In Situ Hybridization, Lac Operon, Macrophages, Male, Membrane Proteins, Mesoderm, Metalloendopeptidases, Mice, Mice, Inbred C57BL, Mice, Knockout, Mice, Transgenic, Models, Genetic, Mutation, Time Factors, Tissue Distribution

ADAM8 (a disintegrin and metalloprotease 8, also referred to as MS2/CD156a) is a membrane-anchored metalloprotease that was first identified in a macrophage cell line and has been implicated in neurodegenerative diseases. Here, we evaluated the expression of ADAM8 during mouse development and generated mice lacking ADAM8 (Adam8-/- mice). During early mouse development, ADAM8 is expressed by maternal cells in the decidua and by trophoblast derivatives of the embryo but not in the derivatives of the inner cell mass. At later stages, prominent expression of ADAM8 is seen in the embryo proper, in the gonadal ridge, thymus, developing cartilage and bone, brain and spinal cord, and in the mesenchyme in close proximity to the branch point between the jugular vein and developing lymphatic vessels. Examination of Adam8-/- mice, however, revealed no major defects in these or other structures during development or in adult tissues and no evident pathological phenotypes.

Alternate JournalDev. Dyn.
PubMed ID15580619
Grant ListP30-CA-08748 / CA / NCI NIH HHS / United States
R01 GM064750-04 / GM / NIGMS NIH HHS / United States
R01 GM65740 / GM / NIGMS NIH HHS / United States