Leading science, pioneering therapies
CRM Publications

Individual axons regulate the myelinating potential of single oligodendrocytes in vivo.

TitleIndividual axons regulate the myelinating potential of single oligodendrocytes in vivo.
Publication TypeJournal Article
Year of Publication2011
AuthorsAlmeida RG, Czopka T, ffrench-Constant C, Lyons DA
Date Published2011 Oct
KeywordsAnimals, Animals, Genetically Modified, Axons, Base Sequence, DNA Primers, Microscopy, Electron, Transmission, Myelin Basic Protein, Myelin Sheath, Oligodendroglia, Zebrafish, Zebrafish Proteins

The majority of axons in the central nervous system (CNS) are eventually myelinated by oligodendrocytes, but whether the timing and extent of myelination in vivo reflect intrinsic properties of oligodendrocytes, or are regulated by axons, remains undetermined. Here, we use zebrafish to study CNS myelination at single-cell resolution in vivo. We show that the large caliber Mauthner axon is the first to be myelinated (shortly before axons of smaller caliber) and that the presence of supernumerary large caliber Mauthner axons can profoundly affect myelination by single oligodendrocytes. Oligodendrocytes that typically myelinate just one Mauthner axon in wild type can myelinate multiple supernumerary Mauthner axons. Furthermore, oligodendrocytes that exclusively myelinate numerous smaller caliber axons in wild type can readily myelinate small caliber axons in addition to the much larger caliber supernumerary Mauthner axons. These data indicate that single oligodendrocytes can myelinate diverse axons and that their myelinating potential is actively regulated by individual axons.

Alternate JournalDevelopment
PubMed ID21880787
PubMed Central IDPMC3177314
Grant List934 / / Multiple Sclerosis Society / United Kingdom
G0601744 / / Medical Research Council / United Kingdom
/ / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom