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Imprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts.

TitleImprinted X-inactivation in extra-embryonic endoderm cell lines from mouse blastocysts.
Publication TypeJournal Article
Year of Publication2005
AuthorsKunath T, Arnaud D, Uy GD, Okamoto I, Chureau C, Yamanaka Y, Heard E, Gardner RL, Avner P, Rossant J
JournalDevelopment
Volume132
Issue7
Pagination1649-61
Date Published2005 Apr
ISSN0950-1991
KeywordsAnimals, Blastocyst, Dosage Compensation, Genetic, Endoderm, Female, Genomic Imprinting, Histones, Mice, X Chromosome
Abstract

The extra-embryonic endoderm lineage plays a major role in the nutritive support of the embryo and is required for several inductive events, such as anterior patterning and blood island formation. Blastocyst-derived embryonic stem (ES) and trophoblast stem (TS) cell lines provide good models with which to study the development of the epiblast and trophoblast lineages, respectively. We describe the derivation and characterization of cell lines that are representative of the third lineage of the blastocyst -extra-embryonic endoderm. Extra-embryonic endoderm (XEN) cell lines can be reproducibly derived from mouse blastocysts and passaged without any evidence of senescence. XEN cells express markers typical of extra-embryonic endoderm derivatives, but not those of the epiblast or trophoblast. Chimeras generated by injection of XEN cells into blastocysts showed exclusive contribution to extra-embryonic endoderm cell types. We used female XEN cells to investigate the mechanism of X chromosome inactivation in this lineage. We observed paternally imprinted X-inactivation, consistent with observations in vivo. Based on gene expression analysis, chimera studies and imprinted X-inactivation, XEN cell lines are representative of extra-embryonic endoderm and provide a new cell culture model of an early mammalian lineage.

DOI10.1242/dev.01715
Alternate JournalDevelopment
PubMed ID15753215