Leading science, pioneering therapies
CRM Publications

Human axial progenitors generate trunk neural crest cells in vitro.

TitleHuman axial progenitors generate trunk neural crest cells in vitro.
Publication TypeJournal Article
Year of Publication2018
AuthorsFrith, Jr T, Granata I, Wind M, Stout E, Thompson O, Neumann K, Stavish D, Heath PR, Ortmann D, Hackland JOs, Anastassiadis K, Gouti M, Briscoe J, Wilson V, Johnson SL, Placzek M, Guarracino MR, Andrews PW, Tsakiridis A
Date Published2018 Aug 10

The neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.

Alternate JournalElife
PubMed ID30095409
PubMed Central IDPMC6101942
Grant ListFP7/2007-2013 agreement no. 602423 (Plurimes) / / Seventh Framework Programme /
Biomedical Science Departmental PhD studentship / / University of Sheffield /
University Research Fellow / / Royal Society /
Iteromics Flagship project / / Ministero dell'Istruzione, dell'Università e della Ricerca /
Plurimes / / Seventh Framework Programme /
BB/P000444/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
RG160249 / / Royal Society /
FC001051 / / Cancer Research UK / United Kingdom
Mr/K011200/1 / / Medical Research Council / United Kingdom
FC001051 / / Medical Research Council / United Kingdom
CCLGA 2016 01 / / Little Princess Trust and Children's Cancer and Leukaemia Group /
BB/J015539/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom
FC001051 / / Wellcome /
Publication institute