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Gβ1γ2 activates phospholipase A2-dependent Golgi membrane tubule formation.

TitleGβ1γ2 activates phospholipase A2-dependent Golgi membrane tubule formation.
Publication TypeJournal Article
Year of Publication2014
AuthorsBechler ME, Brown WJ
JournalFront Cell Dev Biol
Volume2
Issue4
Pagination0004
Date Published2014 Feb 28
ISSN2296-634X
Abstract

Heterotrimeric G proteins transduce the ligand binding of transmembrane G protein coupled receptors into a variety of intracellular signaling pathways. Recently, heterotrimeric Gβγ subunit signaling at the Golgi complex has been shown to regulate the formation of vesicular transport carriers that deliver cargo from the Golgi to the plasma membrane. In addition to vesicles, membrane tubules have also been shown to mediate export from the Golgi complex, which requires the activity of cytoplasmic phospholipase A2 (PLA2) enzyme activity. Through the use of an in vitro reconstitution assay with isolated Golgi complexes, we provide evidence that Gβ1γ2 signaling also stimulates Golgi membrane tubule formation. In addition, we show that an inhibitor of Gβγ activation of PLA2 enzymes inhibits in vitro Golgi membrane tubule formation. Additionally, purified Gβγ protein stimulates membrane tubules in the presence of low (sub-threshold) cytosol concentrations. Importantly, this Gβγ stimulation of Golgi membrane tubule formation was inhibited by treatment with the PLA2 antagonist ONO-RS-082. These studies indicate that Gβ1γ2 signaling activates PLA2 enzymes required for Golgi membrane tubule formation, thus establishing a new layer of regulation for this process.

DOI10.3389/fcell.2014.00004
Alternate JournalFront Cell Dev Biol
PubMed ID25019068
PubMed Central IDPMC4091998
Grant ListR01 GM101027 / GM / NIGMS NIH HHS / United States
R56 DK051596 / DK / NIDDK NIH HHS / United States
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