| Title | Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Robin A, Roberts GA, Kisch J, Sabbadin F, Grogan G, Bruce N, Turner NJ, Flitsch SL |
| Journal | Chem Commun (Camb) |
| Issue | 18 |
| Pagination | 2478-80 |
| Date Published | 2009 May 14 |
| ISSN | 1359-7345 |
| Keywords | Camphor 5-Monooxygenase, Oxidoreductases, Protein Engineering, Protein Structure, Tertiary, Recombinant Fusion Proteins, Rhodococcus, Transformation, Genetic |
| Abstract | A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor. |
| DOI | 10.1039/b901716j |
| Alternate Journal | Chem. Commun. (Camb.) |
| PubMed ID | 19532862 |
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