Title | DNA electrophoresis: historical and theoretical perspectives. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Roberts GA, Dryden DTF |
Journal | Methods Mol Biol |
Volume | 1054 |
Pagination | 1-9 |
Date Published | 2013 |
ISSN | 1940-6029 |
Keywords | DNA, Electrophoresis, Agar Gel, Electrophoresis, Gel, Pulsed-Field, Humans, Sepharose |
Abstract | The technique of gel electrophoresis is now firmly established as a routine laboratory method for analyzing DNA. Here, we describe the development of the methodology as well as a brief explanation of how the technique works. There is a short introduction to pulsed-field agarose gel electrophoresis, which represents a critical advancement in the method that facilitates the analysis of very large fragments of DNA. Finally, theoretical considerations are included. |
DOI | 10.1007/978-1-62703-565-1_1 |
Alternate Journal | Methods Mol. Biol. |
PubMed ID | 23913282 |
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