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Distinct SoxB1 networks are required for naïve and primed pluripotency.

TitleDistinct SoxB1 networks are required for naïve and primed pluripotency.
Publication TypeJournal Article
Year of Publication2017
AuthorsCorsinotti A, Wong FCk, Tatar T, Szczerbinska I, Halbritter F, Colby D, Gogolok S, Pantier R, Liggat K, Mirfazeli ES, Hall-Ponsele E, Mullin NP, Wilson V, Chambers I
Date Published2017 12 19

Deletion offrom mouse embryonic stem cells (ESCs) causes trophectodermal differentiation. While this can be prevented by enforced expression of the related SOXB1 proteins, SOX1 or SOX3, the roles of SOXB1 proteins in epiblast stem cell (EpiSC) pluripotency are unknown. Here, we show thatcan be deleted from EpiSCs with impunity. This is due to a shift in the balance of SoxB1 expression in EpiSCs, which have decreased Sox2 and increased Sox3 compared to ESCs. Consistent with functional redundancy,can also be deleted from EpiSCs without eliminating self-renewal. However, deletion of bothandprevents self-renewal. The overall SOXB1 levels in ESCs affect differentiation choices: neural differentiation ofheterozygous ESCs is compromised, while increased SOXB1 levels divert the ESC to EpiSC transition towards neural differentiation. Therefore, optimal SOXB1 levels are critical for each pluripotent state and for cell fate decisions during exit from naïve pluripotency.

Alternate JournalElife
PubMed ID29256862
PubMed Central IDPMC5758114
Grant List / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
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