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Differential stability of a human mini-chromosome in mouse cell lines.

TitleDifferential stability of a human mini-chromosome in mouse cell lines.
Publication TypeJournal Article
Year of Publication1998
AuthorsLoupart ML, Shen MH, Smith A
Date Published1998 Sep
KeywordsAnimals, Cell Fusion, Centromere, CHO Cells, Chromosomes, Human, Cricetinae, Cricetulus, Electrophoresis, Gel, Pulsed-Field, Genetic Vectors, Humans, L Cells (Cell Line), Mice, Selection, Genetic, Species Specificity

A 4 Mb human mini-chromosome, DeltaDelta2, was transferred from Chinese hamster ovary (CHO) cells into a mouse L cell line. The mini-chromosome could be transferred intact into the L cells, with 112/119 clones maintaining a mini-chromosome of the same size as the original. Ten clones were grown for 30 days in continuous culture. The mini-chromosomes were maintained stably with or without selection at a copy number of 1-2 per cell and none experienced any size alterations, as determined by pulsed-field gel electrophoresis. Thus DeltaDelta2 is structurally and mitotically stable in L cells. This contrasts with results in embryonic stem cells, in which DeltaDelta2 is highly unstable. These findings indicate that established somatic cell lines, such as L cells and CHO cells, have less stringent controls over centromeric function than do normal embryonic cells.

Alternate JournalChromosoma
PubMed ID9745051
Grant List / / Wellcome Trust / United Kingdom