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Differential expression of the mitochondrial gene cytochrome oxidase II in benign and malignant breast tissue.

TitleDifferential expression of the mitochondrial gene cytochrome oxidase II in benign and malignant breast tissue.
Publication TypeJournal Article
Year of Publication1992
AuthorsSharp MG, Adams SM, Walker RA, Brammar WJ, Varley JM
JournalJ Pathol
Volume168
Issue2
Pagination163-8
Date Published1992 Oct
ISSN0022-3417
KeywordsAdenofibroma, Adolescent, Adult, Base Sequence, Breast Neoplasms, DNA, DNA, Mitochondrial, DNA, Neoplasm, Electron Transport Complex IV, Female, Gene Expression, Humans, Middle Aged, Mitochondria
Abstract

Comparative analysis of expression levels of genes in benign and malignant tumours of the breast has been performed. Differential screening of cDNA libraries identified four genes of the mitochondrial genome as being expressed at different levels in the two tissues compared, but further investigations showed that only the gene encoding subunit 2 of cytochrome c oxidase (COII) is expressed at significantly higher levels in carcinomas compared with fibroadenomas. The mitochondrial genes encoding subunits 2 and 4 of NADH dehydrogenase, and subunit 6 of F0F1ATPase, were not found to be differentially expressed in carcinomas and fibroadenomas. All four genes were expressed in the epithelium of human breast carcinomas, as shown by in situ hybridization. The expression level of the COII gene is also correlated with carcinoma grade. No gross alterations to the mitochondrial DNA from these tumours could be detected. The possible implications of these results on the behavioural differences between fibroadenomas and carcinomas are discussed.

DOI10.1002/path.1711680203
Alternate JournalJ. Pathol.
PubMed ID1334139