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Dielectrophoresis: a review of applications for stem cell research.

TitleDielectrophoresis: a review of applications for stem cell research.
Publication TypeJournal Article
Year of Publication2010
AuthorsPethig R, Menachery A, Pells S, de Sousa P
JournalJ Biomed Biotechnol
Date Published2010
KeywordsAnimals, Biomedical Research, Cell Separation, Electrophoresis, Humans, Stem Cells

Dielectrophoresis can discriminate distinct cellular identities in heterogeneous populations, and monitor cell state changes associated with activation and clonal expansion, apoptosis, and necrosis, without the need for biochemical labels. Demonstrated capabilities include the enrichment of haematopoetic stem cells from bone marrow and peripheral blood, and adult stem cells from adipose tissue. Recent research suggests that this technique can predict the ultimate fate of neural stem cells after differentiation before the appearance of specific cell-surface proteins. This review summarises the properties of cells that contribute to their dielectrophoretic behaviour, and their relevance to stem cell research and translational applications.

Alternate JournalJ. Biomed. Biotechnol.
PubMed ID20490279
PubMed Central IDPMC2871555
Grant ListG0300484 / / Medical Research Council / United Kingdom