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Defined conditions for neural commitment and differentiation.

TitleDefined conditions for neural commitment and differentiation.
Publication TypeJournal Article
Year of Publication2003
AuthorsYing Q-L, Smith AG
JournalMethods Enzymol
Date Published2003
KeywordsAnimals, Cell Culture Techniques, Cell Death, Cell Differentiation, Cell Lineage, Cell Separation, Cells, Cultured, Culture Media, Culture Media, Serum-Free, DNA-Binding Proteins, Flow Cytometry, Green Fluorescent Proteins, High Mobility Group Proteins, HMGB Proteins, Luminescent Proteins, Mice, Microscopy, Phase-Contrast, Neurons, Nuclear Proteins, SOXB1 Transcription Factors, Transcription Factors

The efficiency of monolayer differentiation establishes that commitment of ES cells to a neural fate needs neither multicellular aggregation nor extrinsic inducers. The entire process by which pluripotent ES cells acquire neural specification can be visualized and recorded at the level of individual colonies. Furthermore this simple culture system is amenable to cellular and molecular dissection, promising to yield new insights into the mechanism underlying neural determination in mammals and perhaps to deliver the goal of "directed" homogeneous differentiation of ES cells.

Alternate JournalMeth. Enzymol.
PubMed ID14696356