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Decreased Enhancer-Promoter Proximity Accompanying Enhancer Activation.

TitleDecreased Enhancer-Promoter Proximity Accompanying Enhancer Activation.
Publication TypeJournal Article
Year of Publication2019
AuthorsBenabdallah NS, Williamson I, Illingworth RS, Kane L, Boyle S, Sengupta D, Grimes GR, Therizols P, Bickmore WA
JournalMol Cell
Volume76
Issue3
Pagination473-484.e7
Date Published2019 Nov 07
ISSN1097-4164
Abstract

Enhancers can regulate the promoters of their target genes over very large genomic distances. It is widely assumed that mechanisms of enhancer action involve the reorganization of three-dimensional chromatin architecture, but this is poorly understood. The predominant model involves physical enhancer-promoter interaction by looping out the intervening chromatin. However, studying the enhancer-driven activation of the Sonic hedgehog gene (Shh), we have identified a change in chromosome conformation that is incompatible with this simple looping model. Using super-resolution 3D-FISH and chromosome conformation capture, we observe a decreased spatial proximity between Shh and its enhancers during the differentiation of embryonic stem cells to neural progenitors. We show that this can be recapitulated by synthetic enhancer activation, is impeded by chromatin-bound proteins located between the enhancer and the promoter, and appears to involve the catalytic activity of poly (ADP-ribose) polymerase. Our data suggest that models of enhancer-promoter communication need to encompass chromatin conformations other than looping.

DOI10.1016/j.molcel.2019.07.038
Alternate JournalMol. Cell
PubMed ID31494034
PubMed Central IDPMC6838673
Publication institute
CRM