|Title||The culture of mouse embryonic stem cells and formation of embryoid bodies.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Jackson M, A Taylor H, Jones EA, Forrester LM|
|Journal||Methods Mol Biol|
|Keywords||Animals, Cell Aggregation, Cell Culture Techniques, Cell Differentiation, Cercopithecus aethiops, COS Cells, Cryopreservation, Culture Media, Conditioned, Embryonic Stem Cells, Flow Cytometry, Freezing, Leukemia Inhibitory Factor, Mice, Myocytes, Cardiac, Recombinant Proteins, Suspensions|
Embryonic stem (ES) cells are pluripotent cells isolated from the inner cell mass of the pre-implantation blastocyst. They have the capacity to undergo indefinite rounds of self-renewing cell division and differentiate into all the cell lineages of the developing embryo. In suspension culture, ES cells will differentiate into aggregates known as embryoid bodies in a manner similar to the early embryo. This culture system therefore provides a useful model to study the relatively inaccessible stages of mammalian development. We describe methods for the routine maintenance of mouse embryonic stem cells in culture, assays of stem cell self-renewal potential in monolayer culture and the generation of embryoid bodies to study differentiation pathways.
|Alternate Journal||Methods Mol. Biol.|