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The culture of mouse embryonic stem cells and formation of embryoid bodies.

TitleThe culture of mouse embryonic stem cells and formation of embryoid bodies.
Publication TypeJournal Article
Year of Publication2010
AuthorsJackson M, A Taylor H, Jones EA, Forrester LM
JournalMethods Mol Biol
Date Published2010
KeywordsAnimals, Cell Aggregation, Cell Culture Techniques, Cell Differentiation, Cercopithecus aethiops, COS Cells, Cryopreservation, Culture Media, Conditioned, Embryonic Stem Cells, Flow Cytometry, Freezing, Leukemia Inhibitory Factor, Mice, Myocytes, Cardiac, Recombinant Proteins, Suspensions

Embryonic stem (ES) cells are pluripotent cells isolated from the inner cell mass of the pre-implantation blastocyst. They have the capacity to undergo indefinite rounds of self-renewing cell division and differentiate into all the cell lineages of the developing embryo. In suspension culture, ES cells will differentiate into aggregates known as embryoid bodies in a manner similar to the early embryo. This culture system therefore provides a useful model to study the relatively inaccessible stages of mammalian development. We describe methods for the routine maintenance of mouse embryonic stem cells in culture, assays of stem cell self-renewal potential in monolayer culture and the generation of embryoid bodies to study differentiation pathways.

Alternate JournalMethods Mol. Biol.
PubMed ID20204616