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Cell type-specific interactions of transcription factors with a housekeeping promoter in vivo.

TitleCell type-specific interactions of transcription factors with a housekeeping promoter in vivo.
Publication TypeJournal Article
Year of Publication1993
AuthorsStapleton G, Somma MP, Lavia P
JournalNucleic Acids Res
Volume21
Issue10
Pagination2465-71
Date Published1993 May 25
ISSN0305-1048
Keywords3T3 Cells, Animals, Base Sequence, Binding Sites, Carcinoma, Hepatocellular, Cell Line, Cell Nucleus, DNA, Embryo, Mammalian, Fibroblasts, Humans, Liver Neoplasms, Liver Neoplasms, Experimental, Mice, Molecular Sequence Data, Neuroblastoma, Polymerase Chain Reaction, Promoter Regions, Genetic, Rats, Repetitive Sequences, Nucleic Acid, Stem Cells, TATA Box, Transcription Factors, Tumor Cells, Cultured
Abstract

Mammalian housekeeping promoters represent a class of regulatory elements different from those of tissues-specific genes, lacking a TATA box and associated with CG-rich DNA. We have compared the organization of the housekeeping Htf9 promoter in different cell types by genomic footprinting. The sites of in vivo occupancy clearly reflected local combinations of tissue-specific and ubiquitous binding factors. The flexibility of the Htf9 promoter in acting as the target of cell-specific combinations of factors may ensure ubiquitous expression of the Htf9-associated genes.

Alternate JournalNucleic Acids Res.
PubMed ID8389443
PubMed Central IDPMC309548