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Adherent neural stem (NS) cells from fetal and adult forebrain.

TitleAdherent neural stem (NS) cells from fetal and adult forebrain.
Publication TypeJournal Article
Year of Publication2006
AuthorsPollard SM, Conti L, Sun Y, Goffredo D, Smith A
JournalCereb Cortex
Volume16 Suppl 1
Paginationi112-20
Date Published2006 Jul
ISSN1047-3211
KeywordsAging, Animals, Cell Adhesion, Cell Aggregation, Cell Differentiation, Cell Movement, Cell Proliferation, Cells, Cultured, Male, Mice, Nerve Net, Neurons, Prosencephalon, Stem Cells
Abstract

Stable in vitro propagation of central nervous system (CNS) stem cells would offer expanded opportunities to dissect basic molecular, cellular, and developmental processes and to model neurodegenerative disease. CNS stem cells could also provide a source of material for drug discovery assays and cell replacement therapies. We have recently reported the generation of adherent, symmetrically expandable, neural stem (NS) cell lines derived both from mouse and human embryonic stem cells and from fetal forebrain (Conti L, Pollard SM, Gorba T, Reitano E, Toselli M, Biella G, Sun Y, Sanzone S, Ying QL, Cattaneo E, Smith A. 2005. Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol 3(9):e283). These NS cells retain neuronal and glial differentiation potential after prolonged passaging and are transplantable. NS cells are likely to comprise the resident stem cell population within heterogeneous neurosphere cultures. Here we demonstrate that similar NS cell cultures can be established from the adult mouse brain. We also characterize the growth factor requirements for NS cell derivation and self-renewal. We discuss our current understanding of the relationship of NS cell lines to physiological progenitor cells of fetal and adult CNS.

DOI10.1093/cercor/bhj167
Alternate JournalCereb. Cortex
PubMed ID16766697
Grant ListG0300058 / / Medical Research Council / United Kingdom
G0800784 / / Medical Research Council / United Kingdom
G9806702 / / Medical Research Council / United Kingdom