Douglas Colby

Position:

Research technician

Telephone:

0131 651 9500

Biography:

Started career at CGR (Roger Land Building) 1990 as Tissue Culture Technician
Part of team that discovered Nanog gene
Specialise in ES and lesser extent EpiSc cell culture techniques
Lab Techniques include: Southern, FACS, Genotyping

Publications

2019

Endogenous epitope-tagging of , and identifies TET2 as a naïve pluripotency marker. Pantier, Raphaël, Tatar Tülin, Colby Douglas, and Chambers Ian , Life Sci Alliance, 2019 Oct, Volume 2, Issue 5, (2019)

2018

Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation. Festuccia, Nicola, Halbritter Florian, Corsinotti Andrea, Gagliardi Alessia, Colby Douglas, Tomlinson Simon R., and Chambers Ian , EMBO J, 2018 Nov 02, Volume 37, Issue 21, (2018)

2017

Distinct SoxB1 networks are required for naïve and primed pluripotency. Corsinotti, Andrea, Wong Frederick Ck, Tatar Tülin, Szczerbinska Iwona, Halbritter Florian, Colby Douglas, Gogolok Sabine, Pantier Raphaël, Liggat Kirsten, Mirfazeli Elham S., et al. , Elife, 2017 12 19, Volume 6, (2017)

2016

Distinct Contributions of Tryptophan Residues within the Dimerization Domain to Nanog Function. Mullin, Nicholas P., Gagliardi Alessia, Khoa Le Tran Phuc, Colby Douglas, Hall-Ponsele Elisa, Rowe Arthur J., and Chambers Ian , J Mol Biol, 2017 May 19, (2016)

Distinct Signaling Requirements for the Establishment of ESC Pluripotency in Late-Stage EpiSCs. Illich, Damir Jacob, Zhang Miao, Ursu Andrei, Osorno Rodrigo, Kim Kee-Pyo, Yoon Juyong, Araúzo-Bravo Marcos J., Wu Guangming, Esch Daniel, Sabour Davood, et al. , Cell Reports, 2016 Apr 14, Issue 15, p.1-14, (2016)

2015

Selective influence of Sox2 on POU transcription factor binding in embryonic and neural stem cells. Mistri, Tapan Kumar, Devasia Arun George, Chu Lee Thean, Ng Wei Ping, Halbritter Florian, Colby Douglas, Martynoga Ben, Tomlinson Simon R., Chambers Ian, Robson Paul, et al. , EMBO Rep, 2015 Aug 11, (2015)

2013

A direct physical interaction between Nanog and Sox2 regulates embryonic stem cell self-renewal. Gagliardi, Alessia, Mullin Nicholas P., Tan Zi Ying, Colby Douglas, Kousa Anastasia I., Halbritter Florian, Weiss Jason T., Felker Anastasia, Bezstarosti Karel, Favaro Rebecca, et al. , EMBO J, 2013 Aug 14, Volume 32, Issue 16, p.2231-47, (2013)

Reduced Oct4 expression directs a robust pluripotent state with distinct signaling activity and increased enhancer occupancy by Oct4 and Nanog. Karwacki-Neisius, Violetta, Göke Jonathan, Osorno Rodrigo, Halbritter Florian, Ng Jia Hui, Weiße Andrea Y., Wong Frederick, Gagliardi Alessia, Mullin Nicholas P., Festuccia Nicola, et al. , Cell Stem Cell, 2013 May 2, Volume 12, Issue 5, p.531-45, (2013)

2012

OCT4/SOX2-independent Nanog autorepression modulates heterogeneous Nanog gene expression in mouse ES cells. Navarro, Pablo, Festuccia Nicola, Colby Douglas, Gagliardi Alessia, Mullin Nicholas P., Zhang Wensheng, Karwacki-Neisius Violetta, Osorno Rodrigo, Kelly David, Robertson Morag, et al. , EMBO J, 2012 Dec 12, Volume 31, Issue 24, p.4547-62, (2012)

Esrrb is a direct Nanog target gene that can substitute for Nanog function in pluripotent cells. Festuccia, Nicola, Osorno Rodrigo, Halbritter Florian, Karwacki-Neisius Violetta, Navarro Pablo, Colby Douglas, Wong Frederick, Yates Adam, Tomlinson Simon R., and Chambers Ian , Cell Stem Cell, 2012 Oct 5, Volume 11, Issue 4, p.477-90, (2012)

2009

Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells. Tsakiridis, Anestis, Tzouanacou Elena, Rahman Afifah, Colby Douglas, Axton Richard A., Chambers Ian, Wilson Valerie, Forrester Lesley M., and Brickman Joshua M. , Nucleic Acids Res, 2009 Oct, Volume 37, Issue 19, p.e129, (2009)

2008

The pluripotency rheostat Nanog functions as a dimer. Mullin, Nicholas P., Yates Adam, Rowe Arthur J., Nijmeijer Bianca, Colby Douglas, Barlow Paul N., Walkinshaw Malcolm D., and Chambers Ian , Biochem J, 2008 Apr 15, Volume 411, Issue 2, p.227-31, (2008)

2007

Nanog safeguards pluripotency and mediates germline development. Chambers, Ian, Silva Jose, Colby Douglas, Nichols Jennifer, Nijmeijer Bianca, Robertson Morag, Vrana Jan, Jones Ken, Grotewold Lars, and Smith Austin , Nature, 2007 Dec 20, Volume 450, Issue 7173, p.1230-4, (2007)

2006

Nanog retrotransposed genes with functionally conserved open reading frames. Robertson, Morag, Stenhouse Frances H., Colby Douglas, Marland Jamie R. K., Nichols Jennifer, Tweedie Susan, and Chambers Ian , Mamm Genome, 2006 Jul, Volume 17, Issue 7, p.732-43, (2006)

2003

Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Chambers, Ian, Colby Douglas, Robertson Morag, Nichols Jennifer, Lee Sonia, Tweedie Susan, and Smith Austin , Cell, 2003 May 30, Volume 113, Issue 5, p.643-55, (2003)

Search form