David Hay | MRC Centre for Regenerative Medicine

Position:

Group Leader and Professor of Tissue Engineering

Telephone:

0131 651 9500

Biography:

Group leader of the Pluripotent Stem Cell Hepatocyte Development team
Expert in hepatocyte differentiation and in vitro modelling
Over 10 years experience in pluripotent stem cell biology
Experience of spin-out company formation
PhD 2000 and BSc (Hons) 1996, University of St Andrews, UK

Publications

2019

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells. Lucendo-Villarin, Baltasar, Rashidi Hassan, Alhaque Sharmin, Fischer Lena, Meseguer-Ripolles Jose, Wang Yu, O'Farrelly Cliona, Themis Michael, and Hay David C. , J Vis Exp, 2019 Jul 20, Issue 149, (2019)

Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells. Calabrese, Diego, Roma Guglielmo, Bergling Sebastian, Carbone Walter, Mele Valentina, Nuciforo Sandro, Fofana Isabel, Campana Benedetta, Szkolnicka Dagmara, Hay David C., et al. , PLoS One, 2019, Volume 14, Issue 8, p.e0221762, (2019)

2018

Modelling non-alcoholic fatty liver disease in human hepatocyte-like cells. Lyall, Marcus J., Cartier Jessy, Thomson John P., Cameron Kate, Meseguer-Ripolles Jose, O'Duibhir Eoghan, Szkolnicka Dagmara, Villarin Baltasar Lucendo, Wang Yu, Blanco Giovanny Rodriguez, et al. , Philos Trans R Soc Lond B Biol Sci, 2018 Jul 05, Volume 373, Issue 1750, (2018)

Innate immunity in stem cell-derived hepatocytes. Fischer, Lena, Hay David C., and O'Farrelly Cliona , Philos Trans R Soc Lond B Biol Sci, 2018 Jul 05, Volume 373, Issue 1750, (2018)

Developing defined substrates for stem cell culture and differentiation. Hagbard, Louise, Cameron Katherine, August Paul, Penton Christopher, Parmar Malin, Hay David C., and Kallur Therése , Philos Trans R Soc Lond B Biol Sci, 2018 Jul 05, Volume 373, Issue 1750, (2018)

Designer human tissue: coming to a lab near you. Hay, David C., and O'Farrelly Cliona , Philos Trans R Soc Lond B Biol Sci, 2018 Jul 05, Volume 373, Issue 1750, (2018)

Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells. Meseguer-Ripolles, Jose, Lucendo-Villarin Baltasar, Wang Yu, and Hay David C. , J Vis Exp, 2018 07 27, Issue 137, (2018)

3D human liver tissue from pluripotent stem cells displays stable phenotype in vitro and supports compromised liver function in vivo. Rashidi, Hassan, Luu Nguyet-Thin, Alwahsh Salamah M., Ginai Maaria, Alhaque Sharmin, Dong Hua, Tomaz Rute A., Vernay Bertrand, Vigneswara Vasanthy, Hallett John M., et al. , Arch Toxicol, 2018 Oct, Volume 92, Issue 10, p.3117-3129, (2018)

A human iPSC line capable of differentiating into functional macrophages expressing ZsGreen: a tool for the study and tracking of therapeutic cells. Lopez-Yrigoyen, Martha, Fidanza Antonella, Cassetta Luca, Axton Richard A., A Taylor Helen, Meseguer-Ripolles Jose, Tsakiridis Anestis, Wilson Valerie, Hay David C., Pollard Jeffrey W., et al. , Philos Trans R Soc Lond B Biol Sci, 2018 Jul 05, Volume 373, Issue 1750, (2018)

2017

Erratum to: Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells. Lucendo-Villarin, Baltasar, Filis Panagiotis, Swortwood Madeleine J., Huestis Marilyn A., Meseguer-Ripolles Jose, Cameron Kate, Iredale John P., O'Shaughnessy Peter J., Fowler Paul A., and Hay David C. , Arch Toxicol, 2017 Oct 03, (2017)

A Drug-Induced Hybrid Electrospun Poly-Capro-Lactone: Cell-Derived Extracellular Matrix Scaffold for Liver Tissue Engineering. Grant, Rhiannon, Hay David C., and Callanan Anthony , Tissue Eng Part A, 2017 Jul, Volume 23, Issue 13-14, p.650-662, (2017)

Modelling foetal exposure to maternal smoking using hepatoblasts from pluripotent stem cells. Lucendo-Villarin, Baltasar, Filis Panagiotis, Swortwood Madeleine J., Huestis Marilyn A., Meseguer-Ripolles Jose, Cameron Kate, Iredale John P., O'Shaughnesy Peter J., Fowler Paul A., and Hay David C. , Arch Toxicol, 2017 May 16, (2017)

Mechanistic evaluation of primary human hepatocyte culture using global proteomic analysis reveals a selective dedifferentiation profile. Heslop, James A., Rowe Cliff, Walsh Joanne, Sison-Young Rowena, Jenkins Roz, Kamalian Laleh, Kia Richard, Hay David C., Jones Robert P., Malik Hassan Z., et al. , Arch Toxicol, 2017 Jan, Volume 91, Issue 1, p.439-452, (2017)

Stem cell-derived models to improve mechanistic understanding and prediction of human drug-induced liver injury. Goldring, Christopher, Antoine Daniel J., Bonner Frank, Crozier Jonathan, Denning Chris, Fontana Robert J., Hanley Neil A., Hay David C., Ingelman-Sundberg Magnus, Juhila Satu, et al. , Hepatology, 2017 Feb, Volume 65, Issue 2, p.710-721, (2017)

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells. Wang, Yu, Alhaque Sharmin, Cameron Kate, Meseguer-Ripolles Jose, Lucendo-Villarin Baltasar, Rashidi Hassan, and Hay David C. , J Vis Exp, 2017 Mar 02, Issue 121, (2017)

2016

Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering. Lucendo-Villarin, Baltasar, Rashidi H, Cameron Kate, and Hay David C. , J Mater Chem B Mater Biol Med, 2016 May 28, Volume 4, Issue 20, p.3433-3442, (2016)

Mass production of stem cell derived human hepatocytes for experimental medicine. Wang, Yu, and Hay David C. , Expert Rev Gastroenterol Hepatol, 2016 May 9, p.1-3, (2016)

Reducing Hepatocyte Injury and Necrosis in Response to Paracetamol Using Noncoding RNAs. Szkolnicka, Dagmara, Lucendo-Villarin Baltasar, Moore Joanna K., Simpson Kenneth J., Forbes Stuart J., and Hay David C. , Stem Cells Transl Med, 2016 Apr 7, (2016)

Fluid shear stress modulation of hepatocyte-like cell function. Rashidi, H., Alhaque Sharmin, Szkolnicka Dagmara, Flint Oliver, and Hay David C. , Arch Toxicol, 2016 Mar 15, (2016)

Advances in generating hepatocytes from pluripotent stem cells for translational medicine. Szkolnicka, Dagmara, and Hay David C. , Stem Cells, 2016 Mar 26, (2016)

Prospective purification of perivascular presumptive mesenchymal stem cells from human adipose tissue: process optimization and cell population metrics across a large cohort of diverse demographics. West, C C., Hardy W R., Murray I R., James A W., Corselli M, Pang S, Black C, Lobo S E., Sukhija K, Liang P, et al. , Stem Cell Res Ther, 2016, Volume 7, Issue 1, p.47, (2016)

Low-Density Lipoprotein Uptake Demonstrates a Hepatocyte Phenotype in the Dog, but Is Nonspecific Gow, Adam G., Muirhead Rhona, Hay David C., and Argyle David J. , Stem Cells and Development, Jan-12-2016, Volume 25, Issue 1, p.90 - 100, (2016)

2015

Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes. Cameron, Kate, Tan Rosanne, Schmidt-Heck Wolfgang, Campos Gisela, Lyall Marcus J., Wang Yu, Lucendo-Villarin Baltasar, Szkolnicka Dagmara, Bates Nicola, Kimber Susan J., et al. , Stem Cell Reports, 2015 Dec 8, Volume 5, Issue 6, p.1250-62, (2015)

Polarisation and functional characterisation of hepatocytes derived from human embryonic and mesenchymal stem cells Palakkan, Anwar, Drummond Robert, Anderson Richard A., Greenhough Sebastian, Tv Kumary, Hay David C., and Ross James , Biomedical Reports, Jun-06-2016, (2015)

Concise Review: Workshop Review: Understanding and Assessing the Risks of Stem Cell-Based Therapies Heslop, J. A., Hammond T. G., Santeramo I., A. Piella Tort, Hopp I., Zhou J., Baty R., Graziano E. I., B. Marco Proto, Caron A., et al. , Stem Cells Translational Medicine, Jan-04-2015, Volume 4, Issue 4, p.389 - 400, (2015)

Maternal Smoking Dysregulates Protein Expression in Second Trimester Human Fetal Livers in a Sex-Specific Manner Filis, Panagiotis, Nagrath Nalin, Fraser Margaret, Hay David C., Iredale John P., O'Shaughnessy Peter, and Fowler Paul A. , The Journal of Clinical Endocrinology & Metabolism, Jan-06-2015, Volume 100, Issue 6, p.E861 - E870, (2015)

Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells Godoy, Patricio, Schmidt-Heck Wolfgang, Natarajan Karthick, Lucendo-Villarin Baltasar, Szkolnicka Dagmara, Asplund Annika, rquist Petter ö, Widera Agata, ber Regina ö, Campos Gisela, et al. , Journal of Hepatology, Jan-10-2015, Volume 63, Issue 4, p.934 - 942, (2015)

Galectin-3 regulates hepatic progenitor cell expansion during liver injury Hsieh, W.-C., Mackinnon A. C., Lu W.-Y., Jung J., Boulter L., Henderson N. C., Simpson K. J., Schotanus B., Wojtacha D., Bird T. G., et al. , Gut, Jan-02-2015, Volume 64, Issue 2, p.312 - 321, (2015)

Polyurethane: Stable Cell Phenotype Requires Plasticity: Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance (Adv. Healthcare Mater. 12/2015). Villarin, Baltasar Lucendo, Cameron Kate, Szkolnicka Dagmara, Rashidi H., Bates Nicola, Kimber Susan J., Flint Oliver, Forbes Stuart J., Iredale John P., Bradley Mark, et al. , Adv Healthc Mater, 2015 Aug, Volume 4, Issue 12, p.1819, (2015)

Hepatic progenitor cells of biliary origin with liver repopulation capacity. Lu, Wei-Yu, Bird Thomas G., Boulter Luke, Tsuchiya Atsunori, Cole Alicia M., Hay Trevor, Guest Rachel V., Wojtacha Davina, Man Tak Yung, Mackinnon Alison, et al. , Nat Cell Biol, 2015 Jul 20, (2015)

Polymer Supported Directed Differentiation Reveals a Unique Gene Signature Predicting Stable Hepatocyte Performance. Lucendo-Villarin, Baltasar, Cameron Kate, Szkolnicka Dagmara, Rashidi H., Bates Nicola, Kimber Susan J., Flint Oliver, Forbes Stuart J., Iredale John P., Bradley Mark, et al. , Adv Healthc Mater, 2015 Jun 24, (2015)

Discovery of a Novel Polymer for Human Pluripotent Stem Cell Expansion and Multilineage Differentiation. Celiz, Adam D., Smith James G. W., Patel Asha K., Hook Andrew L., Rajamohan Divya, George Vinoj T., Flatt Luke, Patel Minal J., Epa Vidana C., Singh Taranjit, et al. , Adv Mater, 2015 Jun 1, (2015)

2014

Methods in Molecular BiologyProtocols in In Vitro Hepatocyte ResearchSerum-Free Directed Differentiation of Human Embryonic Stem Cells to Hepatocytes Cameron, Kate, Lucendo-Villarin Baltasar, Szkolnicka Dagmara, and Hay David C. , Volume 1250, New York, NY, p.105 - 111, (2014)

Stabilizing hepatocellular phenotype using optimized synthetic surfaces. Lucendo-Villarin, Baltasar, Cameron Kate, Szkolnicka Dagmara, Travers Paul, Khan Ferdous, Walton Jeffrey G., Iredale John, Bradley Mark, and Hay David C. , J Vis Exp, 2014, Issue 91, (2014)

Deriving functional hepatocytes from pluripotent stem cells. Szkolnicka, Dagmara, Farnworth Sarah L., Lucendo-Villarin Baltasar, and Hay David C. , Curr Protoc Stem Cell Biol, 2014, Volume 30, p.1G.5.1-1G.5.12, (2014)

Modulating innate immunity improves hepatitis C virus infection and replication in stem cell-derived hepatocytes. Zhou, Xiaoling, Sun Pingnan, Lucendo-Villarin Baltasar, Angus Allan G. N., Szkolnicka Dagmara, Cameron Kate, Farnworth Sarah L., Patel Arvind H., and Hay David C. , Stem Cell Reports, 2014 Jul 8, Volume 3, Issue 1, p.204-14, (2014)

Current Protocols in Stem Cell BiologyDeriving Functional Hepatocytes from Pluripotent Stem Cells Szkolnicka, Dagmara, Farnworth Sarah L., Lucendo-Villarin Baltasar, and Hay David C. , Hoboken, NJ, USA, p.1G.5.1 - 1G.5.12, (2014)

Accurate prediction of Drug Induced Liver Injury using stem cell-derived populations. Szkolnicka, Dagmara, Farnworth Sarah L., Lucendo-Villarin Baltasar, Storck Christopher, Zhou Wenli, Iredale John P., Flint Oliver, and Hay David C. , Stem Cells Transl Med, 2014 Feb, Volume 3, Issue 2, p.141-8, (2014)

Ethical, legal and practical issues of establishing an adipose stem cell bank for research. West, Christopher C., Murray Iain R., González Z N., Hindle P, Hay David C., Stewart K J., and Péault Bruno , J Plast Reconstr Aesthet Surg, 2014 Jun, Volume 67, Issue 6, p.745-51, (2014)

Generation of functional neurons from feeder-free, keratinocyte-derived equine induced pluripotent stem cells. Sharma, Ruchi, Livesey Matthew Robert, Wyllie David J. A., Proudfoot Christopher, Whitelaw Christopher Bruce Alex, Hay David C., and Donadeu Francesc Xavier , Stem Cells Dev, 2014 Jul 1, Volume 23, Issue 13, p.1524-34, (2014)

Galectin-3 regulates hepatic progenitor cell expansion during liver injury. Hsieh, Wei-Chen, Mackinnon Alison C., Lu Wei-Yu, Jung Jonathan, Boulter Luke, Henderson Neil C., Simpson Kenneth J., Schotanus Baukje, Wojtacha Davina, Bird Tom G., et al. , Gut, 2014 May 16, (2014)

2013

Developing high-fidelity hepatotoxicity models from pluripotent stem cells. Medine, C. N., Lucendo-Villarin Baltasar, Storck Christopher, Wang Faye, Szkolnicka Dagmara, Khan Ferdous, Pernagallo Salvatore, Black James R., Marriage Howard M., Ross James A., et al. , Stem Cells Transl Med, 2013 Jul, Volume 2, Issue 7, p.505-9, (2013)

Rapid and scalable human stem cell differentiation: now in 3D. Hay, David C. , Stem Cells Dev, 2013 Oct 15, Volume 22, Issue 20, p.2691-2, (2013)

Pluripotent stem cell-derived hepatocytes: potential and challenges in pharmacology. Szkolnicka, Dagmara, Zhou Wenli, Lucendo-Villarin Baltasar, and Hay David C. , Annu Rev Pharmacol Toxicol, 2013, Volume 53, p.147-59, (2013)

Development of an embryoid body-based screening strategy for assessing the hepatocyte differentiation potential of human embryonic stem cells following single-cell dissociation. Greenhough, Sebastian, Bradburn Helen, Gardner John, and Hay David C. , Cell Reprogram, 2013 Feb, Volume 15, Issue 1, p.9-14, (2013)

Liver tissue engineering and cell sources: issues and challenges. Palakkan, Anwar A., Hay David C., Kumar P R. Anil, Kumary T V., and Ross James A. , Liver Int, 2013 May, Volume 33, Issue 5, p.666-76, (2013)

2012

Novel biopolymers to enhance endothelialisation of intra-vascular devices. Pernagallo, Salvatore, Tura Olga, Wu Mei, Samuel Kay, Diaz-Mochon Juan Jose, Hansen Anna, Zhang Rong, Jackson Melany, Padfield G J., Hadoke Patrick W. F., et al. , Adv Healthc Mater, 2012 Sep, Volume 1, Issue 5, p.646-56, (2012)

SUMOylation of HNF4α regulates protein stability and hepatocyte function. Zhou, Wenli, Hannoun Zara, Jaffray Ellis, Medine C. N., Black James R., Greenhough Sebastian, Zhu Liang, Ross James A., Forbes Stuart J., Wilmut Ian, et al. , J Cell Sci, 2012 Aug 1, Volume 125, Issue Pt 15, p.3630-5, (2012)

A genome-wide screen in human embryonic stem cells reveals novel sites of allele-specific histone modification associated with known disease loci. Prendergast, James G. D., Tong Pin, Hay David C., Farrington Susan M., and Semple Colin A. M. , Epigenetics Chromatin, 2012, Volume 5, Issue 1, p.6, (2012)

2011

Lineage-specific distribution of high levels of genomic 5-hydroxymethylcytosine in mammalian development. Ruzov, Alexey, Tsenkina Yanina, Serio Andrea, Dudnakova Tatiana, Fletcher Judy, Bai Y, Chebotareva Tatiana, Pells Steve, Hannoun Zara, Sullivan Gareth, et al. , Cell Res, 2011 Sep, Volume 21, Issue 9, p.1332-42, (2011)

Persistence of functional hepatocyte-like cells in immune-compromised mice. Payne, Catherine, Samuel Kay, Pryde Anne, King Jason, Brownstein David, Schrader Joerg, Medine C. N., Forbes Stuart J., Iredale John P., Newsome Philip N., et al. , Liver Int, 2011 Feb, Volume 31, Issue 2, p.254-62, (2011)

Unbiased screening of polymer libraries to define novel substrates for functional hepatocytes with inducible drug metabolism. Hay, David C., Pernagallo Salvatore, Diaz-Mochon Juan Jose, Medine C. N., Greenhough Sebastian, Hannoun Zara, Schrader Joerg, Black James R., Fletcher Judy, Dalgetty Donna, et al. , Stem Cell Res, 2011 Mar, Volume 6, Issue 2, p.92-102, (2011)

2010

The complexities of engineering human stem cell-derived therapeutics. Hay, David C., Ross James A., Gallagher Ronnie, and Bagnaninchi Pierre O. , J Biomed Biotechnol, 2010, Volume 2010, p.654964, (2010)

Role of stem-cell-derived hepatic endoderm in human drug discovery. Medine, C. N., Greenhough Sebastian, and Hay David C. , Biochem Soc Trans, 2010 Aug, Volume 38, Issue 4, p.1033-6, (2010)

Cadaveric hepatocytes repopulate diseased livers: life after death. Hay, David C. , Gastroenterology, 2010 Sep, Volume 139, Issue 3, p.729-31, (2010)

Pluripotent stem cell derived hepatocyte like cells and their potential in toxicity screening. Greenhough, Sebastian, Medine C. N., and Hay David C. , Toxicology, 2010 Dec 30, Volume 278, Issue 3, p.250-5, (2010)

Post-translational modification by SUMO. Hannoun, Zara, Greenhough Sebastian, Jaffray Ellis, Hay Ronald T., and Hay David C. , Toxicology, 2010 Dec 30, Volume 278, Issue 3, p.288-93, (2010)

The comparison between conditioned media and serum-free media in human embryonic stem cell culture and differentiation. Hannoun, Zara, Fletcher Judy, Greenhough Sebastian, Medine C. N., Samuel Kay, Sharma Ruchi, Pryde Anne, Black James R., Ross James A., Wilmut Ian, et al. , Cell Reprogram, 2010 Apr, Volume 12, Issue 2, p.133-40, (2010)

Three-dimensional culture of human embryonic stem cell derived hepatic endoderm and its role in bioartificial liver construction. Sharma, Ruchi, Greenhough Sebastian, Medine C. N., and Hay David C. , J Biomed Biotechnol, 2010, Volume 2010, p.236147, (2010)

Portal venous endothelium in developing human liver contains haematopoietic and epithelial progenitor cells. Terrace, John D., Hay David C., Samuel Kay, Anderson Richard A., Currie Ian S., Parks Rowan W., Forbes Stuart J., and Ross James A. , Exp Cell Res, 2010 May 15, Volume 316, Issue 9, p.1637-47, (2010)

Hepatic endoderm differentiation from human embryonic stem cells. Hannoun, Zara, Filippi Celine, Sullivan Gareth, Hay David C., and Iredale John P. , Curr Stem Cell Res Ther, 2010 Sep, Volume 5, Issue 3, p.233-44, (2010)

Generation of functional human hepatic endoderm from human induced pluripotent stem cells. Sullivan, Gareth, Hay David C., Park In-Hyun, Fletcher Judy, Hannoun Zara, Payne Catherine, Dalgetty Donna, Black James R., Ross James A., Samuel Kay, et al. , Hepatology, 2010 Jan, Volume 51, Issue 1, p.329-35, (2010)

2009

Progress and future challenges in stem cell-derived liver technologies. Dalgetty, Donna, Medine C. N., Iredale John P., and Hay David C. , Am J Physiol Gastrointest Liver Physiol, 2009 Aug, Volume 297, Issue 2, p.G241-8, (2009)

Side population cells in developing human liver are primarily haematopoietic progenitor cells. Terrace, John D., Hay David C., Samuel Kay, Payne Catherine, Anderson Richard A., Currie Ian S., Parks Rowan W., Forbes Stuart J., and Ross James A. , Exp Cell Res, 2009 Aug 1, Volume 315, Issue 13, p.2141-53, (2009)

2008

Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling. Hay, David C., Fletcher Judy, Payne Catherine, Terrace John D., Gallagher Ronald C. J., Snoeys Jan, Black James R., Wojtacha Davina, Samuel Kay, Hannoun Zara, et al. , Proc Natl Acad Sci U S A, 2008 Aug 26, Volume 105, Issue 34, p.12301-6, (2008)

Efficient differentiation of hepatocytes from human embryonic stem cells exhibiting markers recapitulating liver development in vivo. Hay, David C., Zhao Debiao, Fletcher Judy, Hewitt Zoë A., McLean Doris, Urruticoechea-Uriguen Alai, Black James R., Elcombe Cliff, Ross James A., Wolf Roland, et al. , Stem Cells, 2008 Apr, Volume 26, Issue 4, p.894-902, (2008)

The inhibitory role of stromal cell mesenchyme on human embryonic stem cell hepatocyte differentiation is overcome by Wnt3a treatment. Fletcher, Judy, Cui Wei, Samuel Kay, Black James R., Hannoun Zara, Currie Ian S., Terrace John D., Payne Catherine, Filippi Celine, Newsome Philip N., et al. , Cloning Stem Cells, 2008 Sep, Volume 10, Issue 3, p.331-9, (2008)

2007

Progenitor cell characterization and location in the developing human liver. Terrace, John D., Currie Ian S., Hay David C., Masson Neil M., Anderson Richard A., Forbes Stuart J., Parks Rowan W., and Ross James A. , Stem Cells Dev, 2007 Oct, Volume 16, Issue 5, p.771-8, (2007)

Search form

News

Projects