Location: CRM ground floor, room G.43
Access (after training): 24/24h, 7/7 days
Cost (CRM users): £49 peak hours (8am-5pm, week days), £36.75 off-peak, time-lapse session £245
A guide to use this microscope is available here:
sp8_5d_help_guide_for_users.pdf
Specifications
Main features:
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Inverted microscope
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Lasers: 405, 458/476/488/496/514, 561, 594, 633 nm
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Filter-free spectral detection 400-800 nm
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Fast resonant scanner (12kHz)
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2 HyD detectors (45% QE @ 500 nm) and 3 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light
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Fully motorised XY stage for multi-positions, tilling, mosaic …
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galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm)
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Environmental chamber (temperature and 5% CO2)
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Software-controlled water pump for water immersion objectives
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Adaptive Focus Control (AFC) to correct for focus drift
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Advanced imaging techniques: FRAP, FRET, photo-activation, time-lapse, high-content imaging (MatrixScreener module)
- Navigator option
- 3D visualisation module
Objectives (More objectives available upon request at the time of booking)
- 10x, NA 0.40, Air
- 20x, NA 0.75, Air
- 25x, NA 0.95, Water (automatic water immersion)
- 40x, NA 1.1, Water (automatic water immersion)
- 40x, NA 1.25, Oil
- 63x, NA 1.4, Oil
Also available:
- 20x, NA 0.7, multi-immersion (Water, Glycerol, Oil)
Sample carriers:
Slides or chambered-slides
Petri dishes (35 mm, 58 mm)
Multi-well plates
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