A problem that faces many people during imaging is the scattering of light through opaque or semi-opaque thick tissues by lipid membranes: the deeper you go, the less you see. A simple solution is to turn your sample transparent.
The X-Clarity system clears your biological sample in three steps:
- Hydrogel Infusion following fixation (24h)
- Tissue to hydrogel hybridisation (3h)
- Lipid removal in a designated cassette
Specifications
The Facility offers the following cassettes for lipid removal:
- Very Large sample (two rat brain or several adult zebrafish). Well size: 35x53x20mm
- Large samples the size of a mouse brain. Well size: 16.8x16.8x20m
- 48 small samples, the size of a E11 mouse embryo. Well size: 6.5x6.5x10mm
- 192 small samples, the size of a 4 days old zebrafish embryo. Well size: 3x3x10mm
You are then free to stain your tissue appropriately.
Duration of lipid removal is sample dependent: the larger and more lipid rich, the longer it takes.
Clearing is paid by cassette and by clearing time (in hours).
1 hour clearing costs £26 with £2 increment per extra hour clearing time.
Booking:
Send an e-mail to the Facility describing your samples, cost codes. Once your job is accepted, bring your sample to the Facility and we will clear them for you.
Mounting Medium:
For optimal transparency, we recommend that you use the X-Clarity mounting medium which we supply at £120 per 25ml vial
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