Introduction:
Location: SCRM ground floor, room G.45
Access (after training): 24h, 7/7 days
Cost (SCRM users): £24 peak hours (8am-5pm, week days), £18 off-peak, time-lapse session £120
A guide to use this microscope is available here:zeiss_explorer_sop_for_users.pdf
Specifications
Main features: Widefield microscope dedicated to live imaging and deconvolution
- Inverted microscope
- Zeiss Colibri7 LED light source ( LEDs: 385, 475, 555, 590, 630 nm)
- Filter cubes: DAPI, GFP, DsRed, mPlum and Cy5
- Monochrome Hamamatsu flash 4.0 v3 camera
- Colour Zeiss MRc camera
- Motorised XY stage (multi-positions, mosaic)
- Motorised Z-focus
- Pecon incubation chamber with temperature and CO2 controllers (on request)
Filter cubes:
- DAPI (365/65, 395, 480/40)
- GFP (470/40, 495, 525/50)
- dsRed (550/25, 570, 605/70)
- mPlum (587/25, 605, 647/70)
- Cy5 (620/60, 660, 700/75)
Objectives
- 5 x/0.1
- 10x/0.45
- LWD 20x/ 0.5 (for multi-well plates)
- LWD 40x/0.75 (for multi-well plates)
- Plan-Apo 20x/0.8
- Plan-Apo 40x/1 OIL
- Plan-apo 63x/1.4 OIL
Available on request:
- LWD 40x/0.75 (for multi-well plates)
- C-Apo 40x/1.2 Water
Sample carriers:
- Slides or chambered-slides
- Petri dishes (35 mm, 50 mm, 60 mm)
- Multi-well plates