Introduction:
Location: SCRM ground floor, room G.43
Access (after training): 24h, 7/7 days
Cost (SCRM users): £24 peak hours (8am-5pm, week days), £18 off-peak, time-lapse session £120
A basic guide to use this microscope is available here: 
nikon_tie_sop_for_users.pdf
Specifications
Main features: Widefield microscope dedicated to live imaging and deconvolution
- Nikon Eclipse Ti Inverted microscope
- Filter cubes: DAPI, GFP, TxRed, G2-A, Cy5
- Monochrome Hamamatsu Flash4 camera
- Motorised XY stage (multi-positions, mosaic)
- Motorised Z-Focus
- Perfect focus
- Sola light engine
- OKO Incubation chamber with temperature and CO2 controllers
- JOBS module for automated imaging
Objectives
- Plan Fluor 10x/0.3
- S Plan Fluor 20x/0.5 Long Working Distance
- Plan Apo 20x/0.8
- S Plan Fluor 40x/0.6 LWD
- W Apo 40x/1.15 Water (on request)
- Plan Apo 60x/1.40 Oil
Sample carriers:
- Slides or chambered slides
- Petri dishes (35mm, 50mm, 60mm)
- Multiwell plates
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