Introduction:
Location: SCRM ground floor, room G.43
Access (after training): 24/24h, 7/7 days
Cost (SCRM members): £49 peak hours (8am-5pm, week days), £36.75 off-peak, time-lapse session £245
A guide to use this microscope is available here:
sp8_4d_help_guide_for_users.pdf
Specifications
Main features:
- Inverted microscope
- Solid stateLasers: 405, 488, 552 and 638 nm
- Filter-free spectral detection 400-800 nm
- 2 HyD detectors (45% QE @ 500 nm) and 2 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light
- Fully motorised XY stage for multi-positions, tilling, navigator option
- Galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm)
- Digital LightSheet module
Objectives (confocal)
- Obj. HC PL APO 10x/0.40 CS2
- Obj. HC PL APO 20x/0.75 CS2
- Obj. HC Fluotar 25x/0.95 Water long working distance
- Obj. HC PL APO 40x/1.30 Oil CS2
- Obj. HC PL APO 63x/1.40 OIL CS2
Digital LightSheet Illumination objectives:
- L 1.6x/0.05 DLS
- Obj. HC PL FLUOTAR 2.5x/0.07
- HC PL FLUOTAR 5x/0.15
Digital LightSheet TwinFlect mirror:
- DLS TwinFlect 2.5mm
- DLS TwinFlect 5mm
- DLS TwinFlect 7.8mm W
- DLS TwinFlect 7.8mm Gly
Digital LightSheet Collection objectives:
- HC PL FLUOTAR 5x/0.15 IMM DLS
- Obj. HC APO L10x/0.30 W DLS
- Obj. HC FLUOTAR L25x/0.95 W DLS
Digital LightSheet Emission Filters:
- Filter DLS BandPass 455-495
- Filter DLS BandPass 504-545
- Filter DLS BandPass 575-635
- Filter DLS 405/488/561/633 (BandPass 422-474nm, 503-545nm, 582-615nm, 662-750nm)
- Filter DLS 405/488/552/638 (BandPass 420-475nm, 500-540nm, 565-620nm, 655-750nm)
- Filter DLS 458/514 (BandPass 470-500nm, 530-650nm)
- Filter DLS 488/561 (BandPass 504-544nm, 578-707nm
Sample carriers:
- Slides or chambered-slides
- Petri dishes (35 mm, 50 mm)
- Multi-well plates
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