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A wider context for gene trap mutagenesis.

TitleA wider context for gene trap mutagenesis.
Publication TypeJournal Article
Year of Publication2010
AuthorsBrickman JM, Tsakiridis A, To C, Stanford WL
JournalMethods Enzymol
Volume477
Pagination271-95
Date Published2010
ISSN1557-7988
KeywordsAnimals, Cell Line, Embryonic Stem Cells, Genetic Vectors, Mice, Mutagenesis
Abstract

Gene trapping is a technology originally developed for the simultaneous identification and mutation of genes by random integration in embryonic stem (ES) cells. While gene trapping was developed before efficient and high-throughput gene targeting, a significant proportion of the publically available mutant ES cell lines and mice were generated through a number of large-scale gene trapping initiatives. Moreover, elements of gene trap vectors continue to be incorporated into gene targeting vectors as a means to increase the efficiency of homologous recombination. Here, we review the current state of gene trapping technology and the applications of specific types of gene trap vector. As a component of this analysis, we consider the behavior of specific vector types both from the perspective of their application and how they can inform our annotation of the mammalian transcriptome. We consider the utility of gene trap vectors as tools for cell-based expression analysis, targeted screening in embryonic differentiation, and for use in cell lines derived from different lineages.

DOI10.1016/S0076-6879(10)77014-2
Alternate JournalMeth. Enzymol.
PubMed ID20699146
Grant List062965 / / Wellcome Trust / United Kingdom
FRN-36653 / / Canadian Institutes of Health Research / Canada
G0701428 / / Medical Research Council / United Kingdom
G0701429 / / Medical Research Council / United Kingdom
MOP-74528 / / Canadian Institutes of Health Research / Canada