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Vasotocin genes of the teleost fish Catostomus commersoni: gene structure, exon-intron boundary, and hormone precursor organization.

TitleVasotocin genes of the teleost fish Catostomus commersoni: gene structure, exon-intron boundary, and hormone precursor organization.
Publication TypeJournal Article
Year of Publication1990
AuthorsMorley SD, Schönrock C, Heierhorst J, Figueroa J, Lederis K, Richter D
JournalBiochemistry
Volume29
Issue10
Pagination2506-11
Date Published1990 Mar 13
ISSN0006-2960
KeywordsAmino Acid Sequence, Animals, Base Sequence, DNA, Exons, Fishes, Hypothalamus, Introns, Molecular Sequence Data, Oxytocin, Poly A, Polymerase Chain Reaction, Protein Precursors, RNA, Messenger, Vasotocin
Abstract

cDNA clones encoding two members of the vasotocin hormone precursor gene family have been isolated from the white sucker Catostomus commersoni. The hormone is encoded by at least two distinct genes, both of which are expressed, as indicated by Northern blot analysis. Genomic DNA amplified by the polymerase chain reaction has been used to define exon-intron boundaries. Both vasotocin genes contain introns in positions corresponding to those found in the gene of their mammalian counterpart vasopressin. The predicted vasotocin precursors show a surprising degree of sequence divergence, amounting to 45% at the amino acid level, of which only approximately half can be accounted for by conservative amino acid changes. The precursors include a hormone moiety followed by a putative neurophysin sequence that is longer at the C-terminus by a tract of some 30 amino acids by comparison to their mammalian counterpart. Each of these sequences contains a leucine-rich core segment resembling that found in copeptin, a glycopeptide moiety present in mammalian vasopressin precursors.

Alternate JournalBiochemistry
PubMed ID1970742