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An unexpected role for IL-3 in the embryonic development of hematopoietic stem cells.

TitleAn unexpected role for IL-3 in the embryonic development of hematopoietic stem cells.
Publication TypeJournal Article
Year of Publication2006
AuthorsRobin C, Ottersbach K, Durand C, Peeters M, Vanes L, Tybulewicz V, Dzierzak E
JournalDev Cell
Date Published2006 Aug
KeywordsAnimals, Aorta, Cell Proliferation, Cell Survival, Core Binding Factor Alpha 2 Subunit, Embryonic Development, Gonads, Hematopoietic Stem Cells, Interleukin-3, Mesonephros, Mice, Placenta, Yolk Sac

Cytokines are important in adult hematopoiesis, yet their function in embryonic hematopoiesis has been largely unexplored. During development, hematopoietic stem cells (HSCs) are found in the aorta-gonad-mesonephros (AGM) region, yolk sac (YS), and placenta and require the Runx1 transcription factor for their normal generation. Since IL-3 is a Runx1 target and this cytokine acts on adult hematopoietic cells, we examined whether IL-3 affects HSCs in the mouse embryo. Using Runx1 haploinsufficient mice, we show that IL-3 amplifies HSCs from E11 AGM, YS, and placenta. Moreover, we show that IL-3 mutant embryos are deficient in HSCs and that IL-3 reveals the presence of HSCs in the AGM and YS prior to the stage at which HSCs are normally detected. Thus, our studies support an unexpected role for IL-3 during development and strongly suggest that IL-3 functions as a proliferation and/or survival factor for the earliest HSCs in the embryo.

Alternate JournalDev. Cell
PubMed ID16890157
Grant ListG0800784 / / Medical Research Council / United Kingdom
MC_U117527252 / / Medical Research Council / United Kingdom
R01 DK51077 / DK / NIDDK NIH HHS / United States
/ / Wellcome Trust / United Kingdom
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