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Unequal segregation of parental chromosomes in embryonic stem cell hybrids.

TitleUnequal segregation of parental chromosomes in embryonic stem cell hybrids.
Publication TypeJournal Article
Year of Publication2005
AuthorsMatveeva NM, Pristyazhnyuk IE, Temirova SA, Menzorov AG, Vasilkova A, Shilov AG, Smith A, Serov OL
JournalMol Reprod Dev
Date Published2005 Jul
KeywordsAnimals, Cells, Cultured, Chromosomal Instability, Chromosome Segregation, Hybrid Cells, Mice, Polyploidy, Species Specificity, Stem Cells

Chromosome segregation was studied in 14 intra- and 20 inter-specific hybrid clones generated by fusion of Mus musculus embryonic stem (ES) cells with fibroblasts or splenocytes of DD/c mice or Mus caroli. As a control for in vitro evolution of tetraploid karyotype we used a set of hybrid clones obtained by fusion of ES cells (D3) with ES cells (TgTP6.3). Identification of the parental chromosomes in the clones was performed by microsatellite analysis and in situ hybridization with labeled species-specific probes. Both analyses have revealed three types of clones: (i) stable tetraploid, observed only for ES x ES cell hybrids; (ii) bilateral loss of chromosomes of both ES and somatic partners; (iii) unilateral segregation of chromosomes of the somatic partner. Observed unilateral segregation was extensive in ES-splenocyte cell hybrids, but lower in ES-fibroblast hybrid clones. Developmental state of the somatic partner is presumably responsible for directional chromosome loss. Nonrandom segregation implies that initial differences in the parental homologous chromosomes were not immediately equalized implying at least transient persistence of the differentiated epigenotype.

Alternate JournalMol. Reprod. Dev.
PubMed ID15806559
Grant List / / Wellcome Trust / United Kingdom