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TCR-stimulated changes in cell surface CD46 expression generate type 1 regulatory T cells.

TitleTCR-stimulated changes in cell surface CD46 expression generate type 1 regulatory T cells.
Publication TypeJournal Article
Year of Publication2017
AuthorsChoileain SNi, Hay J, Thomas J, Williams AC, Vermeren MM, Benezech C, Gomez-Salazar M, Hugues OR, Vermeren S, Howie SEM, Dransfield I, Astier AL
JournalSci Signal
Date Published2017 Oct 24
KeywordsAdult, CD3 Complex, Female, Glycosylation, Humans, Interferon-gamma, Interleukin-10, Lymphocyte Activation, Male, Membrane Cofactor Protein, Middle Aged, Multiple Sclerosis, Plasmids, Receptors, Antigen, T-Cell, T-Lymphocytes, Regulatory, Th1 Cells

A lack of regulatory T cell function is a critical factor in the pathogenesis of autoimmune diseases, such as multiple sclerosis (MS). Ligation of the complement regulatory protein CD46 facilitates the differentiation of T helper 1 (T1) effector cells into interleukin-10 (IL-10)-secreting type 1 regulatory T cells (Tr1 cells), and this pathway is defective in MS patients. Cleavage of the ectodomain of CD46, which contains three N-glycosylation sites and multiple O-glycosylation sites, enables CD46 to activate T cells. We found that stimulation of the T cell receptor (TCR)-CD3 complex was associated with a reduction in the apparent molecular mass of CD46 in a manner that depended on O-glycosylation. CD3-stimulated changes in CD46 O-glycosylation status reduced CD46 processing and subsequent T cell signaling. During T cell activation, CD46 was recruited to the immune synapse in a manner that required its serine-, threonine-, and proline-rich (STP) region, which is rich in O-glycosylation sites. Recruitment of CD46 to the immune synapse switched T cells from producing the inflammatory cytokine interferon-γ (IFN-γ) to producing IL-10. Furthermore, CD4 T cells isolated from MS patients did not exhibit a CD3-stimulated reduction in the mass of CD46 and thus showed increased amounts of cell surface CD46. Together, these data suggest a possible mechanism underlying the regulatory function of CD46 on T cells. Our findings may explain why this pathway is defective in patients with MS and provide insights into MS pathogenesis that could help to design future immunotherapies.

Alternate JournalSci Signal
PubMed ID29066539
Grant ListMR/M011542/1 / / Medical Research Council / United Kingdom
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