Leading science, pioneering therapies
CRM Publications

A structurally defined mini-chromosome vector for the mouse germ line.

TitleA structurally defined mini-chromosome vector for the mouse germ line.
Publication TypeJournal Article
Year of Publication2000
AuthorsShen MH, Mee PJ, Nichols J, Yang J, Brook F, Gardner RL, Smith AG, Brown WR
JournalCurr Biol
Volume10
Issue1
Pagination31-4
Date Published2000 Jan 13
ISSN0960-9822
KeywordsAnimals, Cell Line, Chimera, Chromosomes, DNA, Recombinant, Embryo Transfer, Female, Fibroblasts, Genetic Vectors, Germ-Line Mutation, Humans, Male, Mice, Mice, Inbred C57BL, Stem Cell Transplantation
Abstract

Yeast artificial mini-chromosomes have helped to define the features of chromosome architecture important for accurate segregation and replication and have been used to identify genes important for chromosome stability and as large-fragment cloning vectors. Artificial chromosomes have been developed in human cells but they do not have defined, experimentally predictable structures. Fragments of human chromosomes have also been introduced into mice and in one case passed through the germ line. In these experiments, however, the structure and sequence organization of the fragments was not defined. Structurally defined mammalian mini-chromosome vectors should allow large tracts of DNA to be introduced into the vertebrate germ line for biotechnological purposes and for investigations of features of chromosome structure that influence gene expression. Here, we have determined the structure and sequence organization of an engineered mammalian mini-chromosome, ST1, and shown that it is stably maintained in vertebrate somatic cells and that it can be transmitted through the mouse germ line.

Alternate JournalCurr. Biol.
PubMed ID10660300