Leading science, pioneering therapies
CRM Publications

Stabilizing hepatocellular phenotype using optimized synthetic surfaces.

TitleStabilizing hepatocellular phenotype using optimized synthetic surfaces.
Publication TypeJournal Article
Year of Publication2014
AuthorsLucendo-Villarin B, Cameron K, Szkolnicka D, Travers P, Khan F, Walton JG, Iredale J, Bradley M, Hay DC
JournalJ Vis Exp
Issue91
Date Published2014
ISSN1940-087X
Abstract

Currently, one of the major limitations in cell biology is maintaining differentiated cell phenotype. Biological matrices are commonly used for culturing and maintaining primary and pluripotent stem cell derived hepatocytes. While biological matrices are useful, they permit short term culture of hepatocytes, limiting their widespread application. We have attempted to overcome the limitations using a synthetic polymer coating. Polymers represent one of the broadest classes of biomaterials and possess a wide range of mechanical, physical and chemical properties, which can be fine-tuned for purpose. Importantly, such materials can be scaled to quality assured standards and display batch-to-batch consistency. This is essential if cells are to be expanded for high through-put screening in the pharmaceutical testing industry or for cellular based therapy. Polyurethanes (PUs) are one group of materials that have shown promise in cell culture. Our recent progress in optimizing a polyurethane coated surface, for long-term culture of human hepatocytes displaying stable phenotype, is presented and discussed.

DOI10.3791/51723
Alternate JournalJ Vis Exp
PubMed ID25285607
Publication institute
CRM