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Serotonin Promotes Development and Regeneration of Spinal Motor Neurons in Zebrafish.

TitleSerotonin Promotes Development and Regeneration of Spinal Motor Neurons in Zebrafish.
Publication TypeJournal Article
Year of Publication2015
AuthorsBarreiro-Iglesias A, Mysiak KS, Scott AL, Reimer MM, Yang Y, Becker CG, Becker T
JournalCell Rep
Volume13
Issue5
Pagination924-32
Date Published2015 Nov 03
ISSN2211-1247
KeywordsAnimals, Interneurons, Motor Neurons, Nerve Regeneration, Neural Stem Cells, Receptor, Serotonin, 5-HT1A, Serotonin, Spinal Cord, Zebrafish
Abstract

In contrast to mammals, zebrafish regenerate spinal motor neurons. During regeneration, developmental signals are re-deployed. Here, we show that, during development, diffuse serotonin promotes spinal motor neuron generation from pMN progenitor cells, leaving interneuron numbers unchanged. Pharmacological manipulations and receptor knockdown indicate that serotonin acts at least in part via 5-HT1A receptors. In adults, serotonin is supplied to the spinal cord mainly (90%) by descending axons from the brain. After a spinal lesion, serotonergic axons degenerate caudal to the lesion but sprout rostral to it. Toxin-mediated ablation of serotonergic axons also rostral to the lesion impaired regeneration of motor neurons only there. Conversely, intraperitoneal serotonin injections doubled numbers of new motor neurons and proliferating pMN-like progenitors caudal to the lesion. Regeneration of spinal-intrinsic serotonergic interneurons was unaltered by these manipulations. Hence, serotonin selectively promotes the development and adult regeneration of motor neurons in zebrafish.

DOI10.1016/j.celrep.2015.09.050
Alternate JournalCell Rep
PubMed ID26565906
PubMed Central IDPMC4635313
Grant ListBB/I01294X/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
Publication institute
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