Title | Prospective identification of myogenic endothelial cells in human skeletal muscle. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Zheng B, Cao B, Crisan M, Sun B, Li G, Logar A, Yap S, Pollett JB, Drowley L, Cassino T, Gharaibeh B, Deasy BM, Huard J, Péault B |
Journal | Nat Biotechnol |
Volume | 25 |
Issue | 9 |
Pagination | 1025-34 |
Date Published | 2007 Sep |
ISSN | 1087-0156 |
Keywords | Adolescent, Adult, Aged, Animals, Antigens, CD56, Biomarkers, Cell Proliferation, Cell Survival, Cells, Cultured, Child, Clone Cells, Endothelial Cells, Flow Cytometry, Humans, Mice, Mice, SCID, Middle Aged, Muscle, Skeletal, Neoplasms, Regeneration, Time Factors |
Abstract | We document anatomic, molecular and developmental relationships between endothelial and myogenic cells within human skeletal muscle. Cells coexpressing myogenic and endothelial cell markers (CD56, CD34, CD144) were identified by immunohistochemistry and flow cytometry. These myoendothelial cells regenerate myofibers in the injured skeletal muscle of severe combined immunodeficiency mice more effectively than CD56+ myogenic progenitors. They proliferate long term, retain a normal karyotype, are not tumorigenic and survive better under oxidative stress than CD56+ myogenic cells. Clonally derived myoendothelial cells differentiate into myogenic, osteogenic and chondrogenic cells in culture. Myoendothelial cells are amenable to biotechnological handling, including purification by flow cytometry and long-term expansion in vitro, and may have potential for the treatment of human muscle disease. |
DOI | 10.1038/nbt1334 |
Alternate Journal | Nat. Biotechnol. |
PubMed ID | 17767154 |
Grant List | 1U54 AR 050733-01 / AR / NIAMS NIH HHS / United States R01 AR 049684 / AR / NIAMS NIH HHS / United States R01 DE 13420-06 / DE / NIDCR NIH HHS / United States |