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PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.

TitlePRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.
Publication TypeJournal Article
Year of Publication2012
AuthorsIllingworth R, Botting CH, Grimes GR, Bickmore WA, Eskeland R
JournalPLoS One
Volume7
Issue4
Paginatione34848
Date Published2012
ISSN1932-6203
KeywordsAnimals, Cell Line, Chromatin, CpG Islands, Embryonic Stem Cells, Genes, Developmental, Genetic Loci, Histones, Mice, Polycomb Repressive Complex 1, Polycomb Repressive Complex 2, Polycomb-Group Proteins, Promoter Regions, Genetic, Repressor Proteins, Ubiquitin-Protein Ligases
Abstract

The essential histone variant H2A.Z localises to both active and silent chromatin sites. In embryonic stem cells (ESCs), H2A.Z is also reported to co-localise with polycomb repressive complex 2 (PRC2) at developmentally silenced genes. The mechanism of H2A.Z targeting is not clear, but a role for the PRC2 component Suz12 has been suggested. Given this association, we wished to determine if polycomb functionally directs H2A.Z incorporation in ESCs. We demonstrate that the PRC1 component Ring1B interacts with multiple complexes in ESCs. Moreover, we show that although the genomic distribution of H2A.Z co-localises with PRC2, Ring1B and with the presence of CpG islands, H2A.Z still blankets polycomb target loci in the absence of Suz12, Eed (PRC2) or Ring1B (PRC1). Therefore we conclude that H2A.Z accumulates at developmentally silenced genes in ESCs in a polycomb independent manner.

DOI10.1371/journal.pone.0034848
Alternate JournalPLoS ONE
PubMed ID22496869
PubMed Central IDPMC3322156
Grant List / / Wellcome Trust / United Kingdom
BB/H008500/1 / / Biotechnology and Biological Sciences Research Council / United Kingdom
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