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Position-dependent plasticity of distinct progenitor types in the primitive streak.

TitlePosition-dependent plasticity of distinct progenitor types in the primitive streak.
Publication TypeJournal Article
Year of Publication2016
AuthorsWymeersch FJ, Huang Y, Blin G, Cambray N, Wilkie R, Wong FCk, Wilson V
JournalElife
Volume5
Date Published2016
ISSN2050-084X
Abstract

The rostrocaudal (head-to-tail) axis is supplied by populations of progenitors at the caudal end of the embryo. Despite recent advances characterising one of these populations, the neuromesodermal progenitors, their nature and relationship to other populations remains unclear. Here we show that neuromesodermal progenitors are a single Sox2(low)T(low) entity whose choice of neural or mesodermal fate is dictated by their position in the progenitor region. The choice of mesoderm fate is Wnt/β-catenin dependent. Wnt/β-catenin signalling is also required for a previously unrecognised phase of progenitor expansion during mid-trunk formation. Lateral/ventral mesoderm progenitors represent a distinct committed state that is unable to differentiate to neural fates, even upon overexpression of the neural transcription factor Sox2. They do not require Wnt/β-catenin signalling for mesoderm differentiation. This information aids the correct interpretation of in vivo genetic studies and the development of in vitro protocols for generating physiologically-relevant cell populations of clinical interest.

DOI10.7554/eLife.10042
Alternate JournalElife
PubMed ID26780186
Grant ListG0802097 / / Medical Research Council / United Kingdom
MR/K011200/1 / / Medical Research Council / United Kingdom
Publication institute
CRM