Leading science, pioneering therapies
CRM Publications

Pluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.

TitlePluripotent stem cell derived hepatocytes: using materials to define cellular differentiation and tissue engineering.
Publication TypeJournal Article
Year of Publication2016
AuthorsLucendo-Villarin B, Rashidi H, Cameron K, Hay DC
JournalJ Mater Chem B Mater Biol Med
Volume4
Issue20
Pagination3433-3442
Date Published2016 May 28
ISSN2050-750X
Abstract

Pluripotent stem cell derived liver cells (hepatocytes) represent a promising alternative to primary tissue for biological and clinical applications. To date, most hepatocyte maintenance and differentiation systems have relied upon the use of animal derived components. This serves as a significant barrier to large scale production and application of stem cell derived hepatocytes. Recently, the use of defined biologics has overcome those limitations in two-dimensional monolayer culture. In order to improve the cell phenotype further, three-dimensional culture systems have been employed to better mimic the in vivo situation, drawing upon materials chemistry, engineering and biology. In this review we discuss efforts in the field, to differentiate pluripotent stem cells towards hepatocytes under defined conditions.

DOI10.1039/c6tb00331a
Alternate JournalJ Mater Chem B Mater Biol Med
PubMed ID27746914
PubMed Central IDPMC5024673
Grant ListMR/K026666/1 / / Medical Research Council / United Kingdom
MR/L010011/1 / / Medical Research Council / United Kingdom
Publication institute
CRM