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Permissiveness to murine leukemia, virus expression during preimplantation and early postimplantation mouse development.

TitlePermissiveness to murine leukemia, virus expression during preimplantation and early postimplantation mouse development.
Publication TypeJournal Article
Year of Publication1990
AuthorsSavatier P, Morgenstern J, Beddington RS
JournalDevelopment
Volume109
Issue3
Pagination655-65
Date Published1990 Jul
ISSN0950-1991
KeywordsAnimals, Blastocyst, Cell Differentiation, Cells, Cultured, Embryonic Development, Female, Gene Expression, Genetic Vectors, Histocytochemistry, Lac Operon, Mice, Moloney murine leukemia virus, Pregnancy, Repetitive Sequences, Nucleic Acid, Retroviridae, Transcription, Genetic
Abstract

Permissiveness to Moloney Murine Leukemia Virus (MoMuLV) expression was examined during preimplantation and early postimplantation development of the mouse embryo. Blastocysts and 8th, 9th and 10th day postimplantation embryos were infected in vitro with a MoMuLV-based retroviral vector expressing the lacZ gene driven off an internal rat beta-actin promoter. Beta-galactosidase-positive cells were identified in all embryonic tissues including inner cell mass, epiblast, mesoderm, endoderm and definitive ectoderm. In contrast, embryos infected with a MoMuLV-based vector expressing the lacZ gene driven off the viral LTR showed beta-galactosidase-positive cells only in mesoderm and definitive ectoderm. We conclude that permissiveness to transcriptional activity of the LTR is acquired immediately upon differentiation of epiblast during gastrulation of the mouse embryo.

Alternate JournalDevelopment
PubMed ID2401217