Title | Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair. |
Publication Type | Journal Article |
Year of Publication | 2016 |
Authors | Hindle P, Baily J, Khan N, Biant LC, A Simpson HR, Péault B |
Journal | Stem Cells Dev |
Volume | 25 |
Issue | 21 |
Pagination | 1659-1669 |
Date Published | 2016 11 01 |
ISSN | 1557-8534 |
Keywords | Animals, Antibodies, Blood Vessels, Cartilage, Articular, Cell Differentiation, Cell Proliferation, Cells, Cultured, Cross Reactions, Disease Models, Animal, Flow Cytometry, Green Fluorescent Proteins, Hydrogel, Polyethylene Glycol Dimethacrylate, Immunohistochemistry, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, Pericytes, Sheep, Transfection, Transplantation, Autologous, Wound Healing |
Abstract | Previous research has indicated that purified perivascular stem cells (PSCs) have increased chondrogenic potential compared to conventional mesenchymal stem cells (MSCs) derived in culture. This study aimed to develop an autologous large animal model for PSC transplantation and to specifically determine if implanted cells are retained in articular cartilage defects. Immunohistochemistry and fluorescence-activated cell sorting were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34-CD45-CD146+) and adventitial cells (CD34+CD45-CD146-). The purified cells demonstrated osteogenic, adipogenic, and chondrogenic potential in culture. Autologous ovine PSCs (oPSCs) were isolated, cultured, and efficiently transfected using a green fluorescence protein (GFP) encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using hydrogel and collagen membranes. Four weeks following implantation, the condyle was explanted and confocal laser scanning microscopy demonstrated the presence of oPSCs in the defect repaired with the hydrogel. These data suggest the testability in a large animal of native MSC autologous grafting, thus avoiding possible biases associated with xenotransplantation. Such a setting will be used in priority for indications in orthopedics, at first to model articular cartilage repair. |
DOI | 10.1089/scd.2016.0165 |
Alternate Journal | Stem Cells Dev. |
PubMed ID | 27554322 |
Grant List | MR/K015710/1 / / Medical Research Council / United Kingdom MR/L012766/1 / / Medical Research Council / United Kingdom |