Leading science, pioneering therapies
CRM Publications

Nuclear transfer to eggs and oocytes.

TitleNuclear transfer to eggs and oocytes.
Publication TypeJournal Article
Year of Publication2011
AuthorsGurdon JB, Wilmut I
JournalCold Spring Harb Perspect Biol
Volume3
Issue6
Date Published2011 Jun
ISSN1943-0264
KeywordsAmphibians, Animals, Cell Dedifferentiation, Cell Differentiation, Cell Nucleus, Epigenesis, Genetic, Gene Expression Regulation, Developmental, Mammals, Mice, Nuclear Transfer Techniques, Oocytes, Xenopus
Abstract

We review experiments in which somatic cell nuclei are transplanted singly to enucleated eggs (metaphase II) in amphibia and mammals and as multiple nuclei to the germinal vesicle of amphibian oocytes (prophase I). These experiments have shown the totipotency of some somatic cell nuclei, as well as switches in cell type and changes in gene expression. Abnormalities of nuclear transplant embryo development increase greatly as nuclei are taken from progressively more differentiated donor cells. The molecular changes that accompany the reprogramming of transplanted nuclei help to indicate the mechanisms used by eggs and oocytes to reprogram gene expression. We discuss the importance of chromosomal protein exchange, of transcription factor supply, and of chromatin access in reprogramming.

DOI10.1101/cshperspect.a002659
Alternate JournalCold Spring Harb Perspect Biol
PubMed ID21555407
PubMed Central IDPMC3098674
Grant List / / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom