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Nanog is the gateway to the pluripotent ground state.

TitleNanog is the gateway to the pluripotent ground state.
Publication TypeJournal Article
Year of Publication2009
AuthorsSilva J, Nichols J, Theunissen TW, Guo G, van Oosten AL, Barrandon O, Wray J, Yamanaka S, Chambers I, Smith A
Date Published2009 Aug 21
KeywordsAdult Stem Cells, Animals, Blastocyst, Cell Dedifferentiation, Embryonic Stem Cells, Female, Germ Layers, Homeodomain Proteins, Mice, Nuclear Reprogramming, X Chromosome

Pluripotency is generated naturally during mammalian development through formation of the epiblast, founder tissue of the embryo proper. Pluripotency can be recreated by somatic cell reprogramming. Here we present evidence that the homeodomain protein Nanog mediates acquisition of both embryonic and induced pluripotency. Production of pluripotent hybrids by cell fusion is promoted by and dependent on Nanog. In transcription factor-induced molecular reprogramming, Nanog is initially dispensable but becomes essential for dedifferentiated intermediates to transit to ground state pluripotency. In the embryo, Nanog specifically demarcates the nascent epiblast, coincident with the domain of X chromosome reprogramming. Without Nanog, pluripotency does not develop, and the inner cell mass is trapped in a pre-pluripotent, indeterminate state that is ultimately nonviable. These findings suggest that Nanog choreographs synthesis of the naive epiblast ground state in the embryo and that this function is recapitulated in the culmination of somatic cell reprogramming.

Alternate JournalCell
PubMed ID19703398
PubMed Central IDPMC3437554
Grant ListG0700665 / / Medical Research Council / United Kingdom
G9806702 / / Medical Research Council / United Kingdom
/ / Biotechnology and Biological Sciences Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom