Leading science, pioneering therapies
CRM Publications

Microsphere-based tracing and molecular delivery in embryonic stem cells.

TitleMicrosphere-based tracing and molecular delivery in embryonic stem cells.
Publication TypeJournal Article
Year of Publication2009
AuthorsTsakiridis A, Alexander LM, Gennet N, Sanchez-Martin RM, Livigni A, Li M, Bradley M, Brickman JM
JournalBiomaterials
Volume30
Issue29
Pagination5853-61
Date Published2009 Oct
ISSN1878-5905
KeywordsAnimals, Biocompatible Materials, Cells, Cultured, Contrast Media, Drug Carriers, Embryonic Stem Cells, Materials Testing, Mice, Microspheres, Proteins, RNA, Small Interfering, Transfection
Abstract

Embryonic stem (ES) cells are in vitro cell lines that can differentiate into all lineages of the fetus and the adult. Despite the versatility of genetic manipulation in murine ES cells, these approaches are time-consuming and rely on inefficient transient cellular delivery systems that can only be applied to undifferentiated ES cell cultures. Here we describe a polystyrene microsphere-based system designed to efficiently deliver biological materials into both undifferentiated and differentiating ES cells. Our results demonstrate that these microspheres can be successfully employed for simultaneous cellular labeling and controlled transfer of various cargos such as fluorophores, proteins and nucleic acids into ES cells without any significant toxicity or loss of pluripotency. This versatile delivery system is also effective in other stem cell lines derived from early embryos, trophoblast and neural stem cells.

DOI10.1016/j.biomaterials.2009.06.024
Alternate JournalBiomaterials
PubMed ID19608269
Grant ListG0701429 / / Medical Research Council / United Kingdom
G117/560 / / Medical Research Council / United Kingdom
/ / Medical Research Council / United Kingdom