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Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos.

TitleMethods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos.
Publication TypeJournal Article
Year of Publication2015
AuthorsHuang Y, Wilkie R, Wilson V
JournalJ Vis Exp
Issue106
Date Published2015
ISSN1940-087X
Abstract

Manipulation and culture of early mouse embryos is a powerful yet largely under-utilized technology enhancing the value of this model system. Conversely, cell culture has been widely used in developmental biology studies. However, it is important to determine whether in vitro cultured cells truly represent in vivo cell types. Grafting cells into embryos, followed by an assessment of their contribution during development is a useful method to determine the potential of in vitro cultured cells. In this study, we describe a method for grafting cells into a defined site of early postimplantation mouse embryos, followed by ex vivo culture. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing precise localization and adjustment of the number of cells receiving exogenous DNA with both high transfection efficiency and low cell death. These techniques, which do not require any specialized equipment, render experimental manipulations of the gastrulation and early organogenesis-stage mouse embryo possible, allowing analysis of commitment in cultured cell subpopulations and the effect of genetic manipulations in situ on cell differentiation.

DOI10.3791/53295
Alternate JournalJ Vis Exp
PubMed ID26780672
Publication institute
CRM