|Title||Maintenance of the pluripotential phenotype of embryonic stem cells through direct activation of gp130 signalling pathways.|
|Publication Type||Journal Article|
|Year of Publication||1994|
|Authors||Yoshida K, Chambers I, Nichols J, Smith A, Saito M, Yasukawa K, Shoyab M, Taga T, Kishimoto T|
|Date Published||1994 Feb|
|Keywords||Amino Acid Sequence, Animals, Antigens, CD, Blastocyst, Cell Differentiation, Cells, Cultured, Chimera, Cytokine Receptor gp130, Female, Gene Expression Regulation, Growth Inhibitors, Interleukin-6, Leukemia Inhibitory Factor, Leukemia Inhibitory Factor Receptor alpha Subunit, Lymphokines, Male, Membrane Glycoproteins, Mice, Mice, Inbred C57BL, Molecular Sequence Data, Phenotype, Receptors, Cytokine, Receptors, Interleukin, Receptors, Interleukin-6, Receptors, OSM-LIF, Signal Transduction, Stem Cells|
Propagation of the undifferentiated pluripotential phenotype of embryonic stem (ES) cells is dependent on the cytokine differentiation inhibiting activity/leukemia inhibitory factor (DIA/LIF). The DIA/LIF receptor complex is a heterodimer of DIA/LIF receptor (DIA/LIF-R) and gp130. The latter is also a component of the interleukin-6 (IL-6) receptor complex. We report that a combination of IL-6 and soluble IL-6 receptor (sIL-6R), which can induce homodimerisation of gp130 and activation of signalling processes, sustains self-renewal of pluripotential ES cells. Our findings indicate that the IL-6/sIL-6R complex acts on ES cells through gp130 alone, bypassing DIA/LIF-R, and therefore implicate gp130 as the key component in the signalling pathway responsible for stem cell renewal.
|Alternate Journal||Mech. Dev.|