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A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.

TitleA lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo.
Publication TypeJournal Article
Year of Publication2014
AuthorsPotts KS, Sargeant TJ, Markham JF, Shi W, Biben C, Josefsson EC, Whitehead LW, Rogers KL, Liakhovitskaia A, Smyth GK, Kile BT, Medvinsky AL, Alexander WS, Hilton DJ, Taoudi S
JournalBlood
Volume124
Issue17
Pagination2725-9
Date Published2014 Oct 23
ISSN1528-0020
Abstract

In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage.

DOI10.1182/blood-2014-02-559468
Alternate JournalBlood
PubMed ID25079356
Publication institute
CRM