Title | A lineage of diploid platelet-forming cells precedes polyploid megakaryocyte formation in the mouse embryo. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Potts KS, Sargeant TJ, Markham JF, Shi W, Biben C, Josefsson EC, Whitehead LW, Rogers KL, Liakhovitskaia A, Smyth GK, Kile BT, Medvinsky AL, Alexander WS, Hilton DJ, Taoudi S |
Journal | Blood |
Volume | 124 |
Issue | 17 |
Pagination | 2725-9 |
Date Published | 2014 Oct 23 |
ISSN | 1528-0020 |
Abstract | In this study, we test the assumption that the hematopoietic progenitor/colony-forming cells of the embryonic yolk sac (YS), which are endowed with megakaryocytic potential, differentiate into the first platelet-forming cells in vivo. We demonstrate that from embryonic day (E) 8.5 all megakaryocyte (MK) colony-forming cells belong to the conventional hematopoietic progenitor cell (HPC) compartment. Although these cells are indeed capable of generating polyploid MKs, they are not the source of the first platelet-forming cells. We show that proplatelet formation first occurs in a unique and previously unrecognized lineage of diploid platelet-forming cells, which develop within the YS in parallel to HPCs but can be specified in the E8.5 Runx1-null embryo despite the absence of the progenitor cell lineage. |
DOI | 10.1182/blood-2014-02-559468 |
Alternate Journal | Blood |
PubMed ID | 25079356 |