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Isolation and manipulation of mouse trophoblast stem cells.

TitleIsolation and manipulation of mouse trophoblast stem cells.
Publication TypeJournal Article
Year of Publication2008
AuthorsHimeno E, Tanaka S, Kunath T
JournalCurr Protoc Stem Cell Biol
VolumeChapter 1
PaginationUnit 1E.4
Date Published2008 Oct
ISSN1938-8969
KeywordsAnimals, Cell Culture Techniques, Cell Line, Cell Separation, Electroporation, Embryonic Stem Cells, Female, Mice, Multipotent Stem Cells, Pregnancy, Transfection, Trophoblasts
Abstract

The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre-implantation blastocysts or from the extraembryonic ectoderm of early post-implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage. This unit describes how to derive, maintain, and manipulate TS cells, including DNA transfection and chimera formation.

DOI10.1002/9780470151808.sc01e04s7
Alternate JournalCurr Protoc Stem Cell Biol
PubMed ID18972374