Title | Isolation and manipulation of mouse trophoblast stem cells. |
Publication Type | Journal Article |
Year of Publication | 2008 |
Authors | Himeno E, Tanaka S, Kunath T |
Journal | Curr Protoc Stem Cell Biol |
Volume | Chapter 1 |
Pagination | Unit 1E.4 |
Date Published | 2008 Oct |
ISSN | 1938-8969 |
Keywords | Animals, Cell Culture Techniques, Cell Line, Cell Separation, Electroporation, Embryonic Stem Cells, Female, Mice, Multipotent Stem Cells, Pregnancy, Transfection, Trophoblasts |
Abstract | The isolation of stable trophoblast stem (TS) cell lines from early mouse embryos has provided a useful cell culture model to study trophoblast development. TS cells are derived from pre-implantation blastocysts or from the extraembryonic ectoderm of early post-implantation embryos. The derivation and maintenance of mouse TS cells is dependent upon continuous fibroblast growth factor (FGF) signaling. Gene expression analysis, differentiation in culture, and chimera formation show that TS cells accurately model the mouse trophoblast lineage. This unit describes how to derive, maintain, and manipulate TS cells, including DNA transfection and chimera formation. |
DOI | 10.1002/9780470151808.sc01e04s7 |
Alternate Journal | Curr Protoc Stem Cell Biol |
PubMed ID | 18972374 |